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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 8

sp. nov., a facultatively anaerobic lignin-degrading bacterium isolated from mangrove sediment Free

Abstract

An alkali lignin-degrading, Gram-stain-negative, rod-shaped, non-motile and facultatively anaerobic bacterium, designated BM_7, was isolated from mangrove sediment of the supralittoral zone in the Jiulong river estuary, PR China. The cells of strain BM_7 were 0.4–0.6 µm wide and 1.0–8.5 µm long. Oxidase and catalase activities were positive. Strain BM_7 could grow at 10–37 °C (optimum, 25–28 °C), at pH 6.0–8.0 (optimum, pH 7.0) and in the presence of 0.5–6 % (w/v) NaCl (optimum, 2%). Phylogenetic analysis of 16S rRNA gene sequences indicated that strain BM_7 belonged to the genus of the family . It showed the highest similarity to JCM 19152 (96.8 %), followed by KCTC 42253 (96.1%). The values of average nucleotide identity and DNA–DNA hybridization were calculated as 76.9, 24.3 and 76.1, 17.4 % between strain BM_7 with JCM 19152 and KCTC 42253, respectively. The major respiratory quinone of strain BM_7 was MK-7. The polar lipids were detected as phosphatidylethanolamine, three unidentified phospholipids and four unidentified aminolipids. The dominant fatty acids consisted of iso-C, anteiso-C, C ω6, iso-C 3-OH, C 6 C 3-OH and C. The genome size of strain BM_7 is 5.6 Mb, with G+C content of 43.4 mol%. Based on the phylogenetic and phenotypic characteristics, strain BM_7 was considered to represent a novel species of the genus , and the name sp. nov. is proposed. The type strain is BM_7 (=MCCC 1A15882=KCTC 72696).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): lignin degradation , mangrove sediment , Mangrovibacterium lignilyticum and taxonomy
Funding
This study was supported by the:
  • Xiamen Ocean Economic Innovation and Development Demonstration Project (Award 16PZP001SF16)
    • Principle Award Recipient: Zongze Shao
  • COMRA program (Award No. DY135-B2-01)
    • Principle Award Recipient: Zongze Shao
  • National Infrastructure of Microbial Resources of China (Award NMRC-2020-9)
    • Principle Award Recipient: Zongze Shao
© 2020 The Authors
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2020-06-29
2025-04-25
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Mangrovibacterium lignilyticum sp. nov., a facultatively anaerobic lignin-degrading bacterium isolated from mangrove sediment
Int J Syst Evol Microbiol 70, 4502 (2020); https://doi.org/10.1099/ijsem.0.004305
/content/journal/ijsem/10.1099/ijsem.0.004305
/content/journal/ijsem/10.1099/ijsem.0.004305
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