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Volume 70, Issue 7

sp. nov., a planctomycete isolated from a deep-sea water sample of the Northwest Indian Ocean Free

Abstract

A Gram-stain-negative, stalked, oval-shaped and budding bacterial strain, designated E7, was isolated from a deep-sea water sample collected from the Northwest Indian Ocean. The novel strain was strictly aerobic, and catalase- and oxidase-positive. It grew at 6–40 °C (optimum 30 °C) and pH 5.5–8.0 (optimum pH 7.0–7.5). The strain required 0.5–9.0 % (w/v) NaCl (optimum 3.0–5.0 %) for growth. Aesculin, starch, pectin and Tween 20 were hydrolysed. Based on 16S rRNA gene sequence analysis, strain E7 showed the highest similarity with DSM 8797 (97.5 %). The average nucleotide identity and DNA–DNA hybridization values between strain E7 and DSM 8797 were 78.0 and 19.3 %, respectively. The predominant cellular fatty acids of strain E7 were C and summed feature 3 (comprising Cω7 and/or Cω6). The major respiratory quinone was menaquinone-6 (MK-6) and the major polar lipids were phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PMME), phosphatidyldimethylethanolamine (PDME), phosphatidylcholine (PC) and diphosphatidylglycerol (DPG). The genomic DNA G+C content of strain E7 was 52.8 mol%. On the basis of phylogenetic inference and phenotypic characteristics, it is proposed that strain E7 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is E7 (=CGMCC 1.16119=KCTC 72737).

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Keyword(s): deep-sea water , Gimesia benthica , phylogenetic analysis and planctomycetes
Funding
This study was supported by the:
  • Senior User Project of RV KEXUE (Award KEXUE2019GZ05 and KEXUE2019G03)
  • National Natural Science Foundation of China (Award 91951105)
    • Principle Award Recipient: Xin Dai
  • China Ocean Mineral Resources R&D Association (Award DY135-B-02)
    • Principle Award Recipient: Li Huang
© 2020 The Authors
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2020-06-26
2024-09-15
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Gimesia benthica sp. nov., a planctomycete isolated from a deep-sea water sample of the Northwest Indian Ocean
Int J Syst Evol Microbiol 70, 4384 (2020); https://doi.org/10.1099/ijsem.0.004301
/content/journal/ijsem/10.1099/ijsem.0.004301
/content/journal/ijsem/10.1099/ijsem.0.004301
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