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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 7

sp. nov., isolated from deep-sea sediment of the Pacific Ocean Free

Abstract

A Gram-strain-negative, rod-shaped, aerobic bacterium, designated 216_PA32_1, was isolated from deep-sea sediment of the Pacific Ocean. Cells of strain 216_PA32_1 were non-motile, oxidase-positive and catalase-negative. The strain could grow at temperatures of 10–45 °C (optimum, 32–35 °C), at pH 5.0–10.0 (optimum, 6.0–7.0) and at salinities of 0–10% (optimum, 2–8%). The principal fatty acid (>10 %) was summed feature 8 (C 6/7). The sole respiratory quinone was Q10 (100 %). The polar lipids included phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, two unidentified phospholipids and five unidentified aminolipids. The G+C content of the chromosomal DNA was 66.3 mol%. According to the 16S rRNA gene similarity, strain 216_PA32_1 showed the highest sequence similarity to JLT 1210 (97.3 %), followed by SS011B1-20 (97.1 %). Phylogenetic trees indicated that strain 216_PA32_1 clustered with strain SS011B1-20. The average nucleotide identity and the DNA–DNA hybridization values between strain 216_PA32_1 and all species of the genus were below 79.5 and 20.6%, respectively. A combination of the phylogenetic, phenotypic, chemotaxonomic and genomic evidence demonstrated that strain 216_PA32_1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 216_PA32_1 (=MCCC 1A14128=KCTC 72688).

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Keyword(s): deep-sea sediment , Pacific Ocean , Pseudooceanicola pacificus sp. nov. and taxonomy
Funding
This study was supported by the:
  • COMRA program (Award DY135-B2-01)
    • Principle Award Recipient: Zongze Shao
  • the Key Research Program of Frontier Sciences of the Chinese Academy of Sciences (Award QYZDJ-SSW-DQC018-02)
    • Principle Award Recipient: Zhiqiang Yu
  • Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program (Award 2017BT01Z134)
    • Principle Award Recipient: Zhiqiang Yu
© 2020 The Authors
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2020-06-24
2025-05-21
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Pseudooceanicola pacificus sp. nov., isolated from deep-sea sediment of the Pacific Ocean
Int J Syst Evol Microbiol 70, 4372 (2020); https://doi.org/10.1099/ijsem.0.004298
/content/journal/ijsem/10.1099/ijsem.0.004298
/content/journal/ijsem/10.1099/ijsem.0.004298
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