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Abstract

A Gram-stain-negative bacterium, designated strain 40Bstr34, isolated from a sediment sample from the West Pacific Ocean, was taxonomically characterized by using a polyphasic approach. The strain was phylogenetically close to LZB041 and CBS5Q-3, with 16S rRNA gene sequence similarities of 98.5 and 97.1 %, respectively. The genome of strain 40Bstr34 featured a G+C content of 65.7 % for a 5.8 Mb chromosome. Up-to-date bacterial core gene set analysis revealed that strain 40Bstr34 represents one independent lineage with LZB041. DNA–DNA hybridization values between strain 40Bstr34 and its phylogenetic neighbours ranged from 30.3–34.2 %, below the cutoff of 70 %. In addition, the corresponding average nucleotide identity values were between 81.8–83.7 %, which are lower than 95 % threshold. The predominant cellular fatty acids of strain 40Bstr34 were summed feature 8 (C ω and/or C ω), cyclo-C ω8 and iso-C 3-OH, and ubiquinone-10 as the predominant respiratory quinone. The major polar lipids included phosphatidylethanolamine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids and two unidentified lipids. Based on the results of phenotypic, chemotaxonomic and genetic analyses, strain 40Bstr34 is identified as representing a novel species of the genus for which the name sp. nov. is proposed. The type strain is 40Bstr34 (=JCM 33903=MCCC 1K04569).

Funding
This study was supported by the:
  • Key Science and Technology Program of Ningbo City (Award 2012C10038)
    • Principle Award Recipient: Jifang Yang
  • Scientific Research Fund of the Second Institute of Oceanography, MNR (Award JG1418)
    • Principle Award Recipient: Jigang Chen
  • Zhejiang Provincial Top Key Discipline of Biological Engineering (Award CX2018019)
    • Principle Award Recipient: Zehao Xue
  • China Ocean Mineral Resources Research and Development Association (Award DY135-E2-02-07)
    • Principle Award Recipient: Jigang Chen
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2020-06-25
2021-10-25
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