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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 7

sp. nov., an actinobacterium isolated from freshwater sediment Free

Abstract

A novel, facultatively anaerobic actinobacterium, designated strain CBA3103, was isolated from sediment of the Geum River in South Korea. Phylogenetic analysis indicated that strain CBA3103 is most closely related to LZ-22 (98.47 % 16S rRNA gene sequence similarity). The genome of strain CBA3103 was 3 649 865 bp with a DNA G+C content of 69.6 mol%. The average nucleotide identity value between strain CBA3103 and LZ-22 was 79.22 %. Cells of strain CBA3103 were Gram-positive, rod-shaped, 0.6–0.9 µm wide and 1.4–2.4 µm long. Growth occurred at 15–40 °C (optimum, 35 °C), at pH 6.0–7.0 (optimum, pH 7.0) and with 0–2 % NaCl (w/v) (optimum, 0–1 %, w/v). The major cellular fatty acids in strain CBA3103 were anteiso-C, anteiso-C A and iso-C. The major respiratory quinone was menaquinone-9(H). The polar lipids of strain CBA3103 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, five unidentified glycolipids and three unidentified phospholipids. Based on the genotypic, phenotypic and chemotaxonomic analyses, strain CBA3103 represents a novel species of the genus , for which the name sp. nov. (type strain CBA3103=KACC 21446=DSM 110288) is proposed.

  • Received:
  • Accepted:
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Keyword(s): actinobacteria , Raineyella fluvialis and taxonomy
Funding
This study was supported by the:
  • Ewha Womans University (KR) (Award Ewha Womans University Research Grant of 2019)
    • Principle Award Recipient: Jin-Kyu Rhee
  • National Research Foundation of Korea (Award 2018R1D1A1B07045349)
    • Principle Award Recipient: Jin-Kyu Rhee
  • National Research Foundation of Korea (Award 2018R1D1A1A09082921)
    • Principle Award Recipient: Seong Woon Roh
  • World Institute of Kimchi (Award KE2001-2)
    • Principle Award Recipient: Seong Woon Roh
  • Nakdonggang National Institute of Biological Resources (Award NNIBR201901213)
    • Principle Award Recipient: Seong Woon Roh
© 2020 The Authors
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2020-06-26
2025-01-25
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Raineyella fluvialis sp. nov., an actinobacterium isolated from freshwater sediment
Int J Syst Evol Microbiol 70, 4298 (2020); https://doi.org/10.1099/ijsem.0.004289
/content/journal/ijsem/10.1099/ijsem.0.004289
/content/journal/ijsem/10.1099/ijsem.0.004289
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