1887

Abstract

A novel marine Gram-stain-negative, non-motile, aerobic and rod-shaped bacterium, designated as strain MT-229, was isolated from the deep seawater in the Mariana Trench and characterized phylogenetically and phenotypically. Bacterial optimal growth occurred at 30 °C (ranging 10–40 °C), pH 6 (ranging 3–11) and with 11 % (w/v) NaCl (ranging 0–17 %). Strain MT-229 was a piezophile, growing optimally at 20 MPa (range 0.1–70 MPa). The nearest phylogenetic neighbours were CGMCC 1.2174 and JCM 17757 with 16S rRNA gene similarity of 98.7 %. The sole respiratory quinone was menaquinone-6 (MK-6). The major polar lipids were phosphatidylethanolamine (PE), two unidentified aminolipids (AL) and ten unidentified lipids. The major fatty acids of strain MT-229 were iso-C, iso-C 3-OH and iso-C G. The G+C content of the genomic DNA was 45.6 mol%. The combined genotypic and phenotypic data indicated that strain MT-229 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain MT-229 (=DSM 109894=MCCC 1K04201). In addition, the whole-genome-based comparisons revealed that the type strains of and belong to a single species. It is, therefore, proposed that be recognized as a heterotypic synonym of .

Funding
This study was supported by the:
  • National Natural Science Foundation of China (CN) (Award 41773069)
    • Principle Award Recipient: Jiasong Fang
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004288
2020-06-24
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/70/7/4315.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.004288&mimeType=html&fmt=ahah

References

  1. Bruns A, Rohde M, Berthe-Corti L. Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 2001; 51:1997–2006 [View Article][PubMed]
    [Google Scholar]
  2. Parte AC. LPSN - List of Prokaryotic names with Standing in Nomenclature (bacterio.net), 20 years on. Int J Syst Evol Microbiol 2018; 68:1825–1829 [View Article][PubMed]
    [Google Scholar]
  3. Kim JM, Jin HM, Jeon CO. Muricauda taeanensis sp. nov., isolated from a marine tidal flat. Int J Syst Evol Microbiol 2013; 63:2672–2677 [View Article][PubMed]
    [Google Scholar]
  4. Lee S-Y, Park S, Oh T-K, Yoon J-H. Muricauda beolgyonensis sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 2012; 62:1134–1139 [View Article][PubMed]
    [Google Scholar]
  5. Yoon J-H, Kang S-J, Jung Y-T, Oh T-K. Muricauda lutimaris sp. nov., isolated from a tidal flat of the Yellow Sea. Int J Syst Evol Microbiol 2008; 58:1603–1607 [View Article][PubMed]
    [Google Scholar]
  6. Liu S-Q, Sun Q-L, Sun Y-Y, Yu C, Sun L. Muricauda iocasae sp. nov., isolated from deep sea sediment of the South China Sea. Int J Syst Evol Microbiol 2018; 68:2538–2544 [View Article][PubMed]
    [Google Scholar]
  7. Yang C, Li Y, Guo Q, Lai Q, Wei J et al. Muricauda zhangzhouensis sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 2013; 63:2320–2325 [View Article][PubMed]
    [Google Scholar]
  8. Dang Y-R, Sun Y-Y, Sun L-L, Yuan X-X, Li Y et al. Muricauda nanhaiensis sp. nov., isolated from seawater of the South China Sea. Int J Syst Evol Microbiol 2019; 69:2089–2094 [View Article][PubMed]
    [Google Scholar]
  9. Zhang X, Liu X, Lai Q, Du Y, Sun F et al. Muricauda indica sp. nov., isolated from deep sea water. Int J Syst Evol Microbiol 2018; 68:881–885 [View Article][PubMed]
    [Google Scholar]
  10. Wang Y, Yang X, Liu J, Wu Y, Zhang X-H. Muricauda lutea sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2017; 67:1064–1069 [View Article][PubMed]
    [Google Scholar]
  11. Zhang Z, Gao X, Qiao Y, Wang Y, Zhang X-H. Muricauda pacifica sp. nov., isolated from seawater of the South Pacific Gyre. Int J Syst Evol Microbiol 2015; 65:4087–4092 [View Article][PubMed]
    [Google Scholar]
  12. Hwang CY, Kim MH, Bae GD, Zhang GI, Kim YH et al. Muricauda olearia sp. nov., isolated from crude-oil-contaminated seawater, and emended description of the genus Muricauda. Int J Syst Evol Microbiol 2009; 59:1856–1861 [View Article][PubMed]
    [Google Scholar]
  13. Mu D-S, Liang Q-Y, Wang X-M, Lu D-C, Shi M-J et al. Metatranscriptomic and comparative genomic insights into resuscitation mechanisms during enrichment culturing. Microbiome 2018; 6:230 [View Article][PubMed]
    [Google Scholar]
  14. Gürtler V, Stanisich VA. New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology 1996; 142 (Pt 1:3–16 [View Article][PubMed]
    [Google Scholar]
  15. Kim O-S, Cho Y-J, Lee K, Yoon S-H, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  16. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. Dna-Dna hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article][PubMed]
    [Google Scholar]
  17. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article][PubMed]
    [Google Scholar]
  18. Wayne L, Brenner DJ, Colwell RR, Grimont PAD, Kandler O. International Committee on systematic bacteriology. Report of the AD hoc Committee on reconciliation of approaches to bacterial Systematics. Int J Syst Bacteriol 1987; 37:463–464
    [Google Scholar]
  19. Thompson CC, Chimetto L, Edwards RA, Swings J, Stackebrandt E et al. Microbial genomic taxonomy. BMC Genomics 2013; 14:913 [View Article][PubMed]
    [Google Scholar]
  20. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 2009; 106:19126–19131 [View Article][PubMed]
    [Google Scholar]
  21. Meier-Kolthoff JP, Göker M. TYGS is an automated high-throughput platform for state-of-the-art genome-based taxonomy. Nat Commun 2019; 10:2182 [View Article][PubMed]
    [Google Scholar]
  22. Wei Y, Fang J, Xu Y, Zhao W, Cao J. Corynebacterium hadale sp. nov. isolated from hadopelagic water of the New Britain Trench. Int J Syst Evol Microbiol 2018; 68:1474–1478 [View Article][PubMed]
    [Google Scholar]
  23. Tindall B, Sikorski J, Smibert R, Krieg N. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM. (editors) In Methods for General and Molecular Microbiology. Edited by, 3rd ed. Washington, DC: American Society for Microbiology; 2007 pp 330–393
    [Google Scholar]
  24. Skerman V. A Guide to the Identification of the Genera of Bacteria: with Methods and Digests of Generic Characteristics, 2nd edn. Baltimore, MD: Williams & Wilkins; 1967
    [Google Scholar]
  25. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  26. YH W, PS Y, Zhou YD, Xu L, Wang CS et al. Muricauda antarctica sp. nov., a marine member of the Flavobacteriaceae isolated from Antarctic seawater. Int J Syst Evol Microbiol 2013; 63:3451–3456
    [Google Scholar]
  27. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  28. Wei Y, Cao J, Yao H, Mao H, Zhu K et al. Paracoccus sediminilitoris sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2019; 69:1035–1040 [View Article][PubMed]
    [Google Scholar]
  29. Wu Y-H, Yu P-S, Zhou Y-D, Xu L, Wang C-S et al. Muricauda antarctica sp. nov., a marine member of the Flavobacteriaceae isolated from Antarctic seawater. Int J Syst Evol Microbiol 2013; 63:3451–3456 [View Article][PubMed]
    [Google Scholar]
  30. Tindall BJ. Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 1990; 66:199–202 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004288
Loading
/content/journal/ijsem/10.1099/ijsem.0.004288
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error