1887

Abstract

A collection of fungal isolates obtained from crop plants, specifically grapevine and blueberry, in Peru were characterised through morphological and DNA sequence analyses of the nuclear ribosomal internal transcribed spacer (ITS), beta-tubulin () and translation elongation factor 1-alpha (α) regions. Isolates produced monomorphic and dimorphic conidiophores typical of members of the genus . Single- and multi-locus gene phylogenies confirmed the isolates as representing members of the genus , more closely related to species in the subgenus . In phylogenetic analyses the isolates grouped in two separate clades, one corresponding to the species and the other one distinct from all known species of the genus . These isolates are recognized as representing a novel species species for which the name is proposed.

Keyword(s): Bionectriaceae , fungi , grapevine and taxonomy
Funding
This study was supported by the:
  • Fundação para a Ciência e a Tecnologia (Award SFRH/BD/129020/2017)
    • Principle Award Recipient: Micael Gonçalves
  • Fundação para a Ciência e a Tecnologia (Award UIDB/50017/2020+UIDP/50017/2020)
    • Principle Award Recipient: Not Applicable
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004286
2020-06-24
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/70/7/4321.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.004286&mimeType=html&fmt=ahah

References

  1. Schroers H. A monograph of Bionectria and its Clonostachys anamorphs. Stud Mycol 2001; 46:1–96
    [Google Scholar]
  2. Toledo AV, Virla E, Humber RA, Paradell SL, Lastra CCL. First record of Clonostachys rosea (Ascomycota: Hypocreales) as an entomopathogenic fungus of Oncometopia tucumana and Sonesimia grossa (Hemiptera: Cicadellidae) in Argentina. J Invertebr Pathol 2006; 92:7–10 [View Article]
    [Google Scholar]
  3. Zhang L, Yang J, Niu Q, Zhao X, Ye F et al. Investigation on the infection mechanism of the fungus Clonostachys rosea against nematodes using the green fluorescent protein. Appl Microbiol Biotechnol 2008; 78:983–990 [View Article][PubMed]
    [Google Scholar]
  4. Moreira GM, Abreu LM, Carvalho VG, Schroers H-J, Pfenning LH. Multilocus phylogeny of Clonostachys subgenus Bionectria from Brazil and description of Clonostachys chloroleuca sp. nov. Mycol Prog 2016; 15:1031–1039 [View Article]
    [Google Scholar]
  5. Rossman AY, Seifert KA, Samuels GJ, Minnis AM, Schroers H-J et al. Genera in Bionectriaceae, Hypocreaceae, and Nectriaceae (Hypocreales) proposed for acceptance or rejection. IMA Fungus 2013; 4:41–51 [View Article][PubMed]
    [Google Scholar]
  6. Schroers H-J, Samuels GJ, Seifert KA, Gams W. Classification of the mycoparasite Gliocladium roseum in Clonostachys as C. rosea, its relationship to Bionectria ochroleuca, and notes on other Gliocladium-like fungi. Mycologia 1999; 91:365–385 [View Article]
    [Google Scholar]
  7. Rodríguez-Gálvez E, Hilário S, Lopes A, Alves A. Diversity and pathogenicity of Lasiodiplodia and Neopestalotiopsis species associated with stem blight and dieback of blueberry plants in Peru. Eur J Plant Pathol 2020; 157:89–102 [View Article]
    [Google Scholar]
  8. Möller EM, Bahnweg G, Sandermann H, Geiger HH. A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic Acids Res 1992; 20:6115–6116 [View Article][PubMed]
    [Google Scholar]
  9. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal genes for phylogenies. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications California: Academic Press; 1990 pp 315–322
    [Google Scholar]
  10. Alves A, Correia A, Luque J, Phillips A. Botryosphaeria corticola, sp. nov. on Quercus species, with notes and description of Botryosphaeria stevensii and its anamorph, Diplodia mutila . Mycologia 2004; 96:598–613 [View Article][PubMed]
    [Google Scholar]
  11. Alves A, Crous PW, Correia A, Phillips AJL. Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae . Fungal Divers 2008; 2008:1–13
    [Google Scholar]
  12. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 1995; 61:1323–1330 [View Article][PubMed]
    [Google Scholar]
  13. O'Donnell K, Cigelnik E. Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol Phylogenet Evol 1997; 7:103–116 [View Article][PubMed]
    [Google Scholar]
  14. Lopes A, Phillips AJL, Alves A. Mating type genes in the genus Neofusicoccum: Mating strategies and usefulness in species delimitation. Fungal Biol 2017; 121:394–404 [View Article][PubMed]
    [Google Scholar]
  15. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  16. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 1999; 41:95–98
    [Google Scholar]
  17. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  18. Swofford DL. PAUP*: Phylogenetic analysis using parsimony (* and other methods). Version 4.0 Sunderland, Massachusetts: Sinauer Associates; 2003
    [Google Scholar]
  19. Ronquist F, Huelsenbeck JP. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 2003; 19:1572–1574 [View Article][PubMed]
    [Google Scholar]
  20. Page RD. TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 1996; 12:357–358 [View Article][PubMed]
    [Google Scholar]
  21. Rayner RW. A Mycological Color Chart Kew: Commonwealth Mycological Institute; 1970
    [Google Scholar]
  22. Mejía LC, Rojas EI, Maynard Z, Bael SV, Arnold AE et al. Endophytic fungi as biocontrol agents of Theobroma cacao pathogens. Biological Control 2008; 46:4–14 [View Article]
    [Google Scholar]
  23. Vaz ABM, Fonseca PLC, Badotti F, Skaltsas D, Tomé LMR et al. A multiscale study of fungal endophyte communities of the foliar endosphere of native rubber trees in eastern Amazon. Sci Rep 2018; 8:16151 [View Article][PubMed]
    [Google Scholar]
  24. Hirooka Y, Kobayashi T. Taxonomic studies of nectrioid fungi in Japan. II: the genus Bionectria. Mycoscience 2007; 48:81–89 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004286
Loading
/content/journal/ijsem/10.1099/ijsem.0.004286
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error