1887

Abstract

Two yellow-pigmented, Gram-stain-negative, aerobic, rod-shaped bacteria were isolated from the water of the hypersaline Chaka Salt Lake (strain SaA2.12) and sediment of Qinghai Lake (strain LaA7.5), PR China. According to the 16S rRNA phylogeny, the isolates belong to the genus , showing the highest 16S rRNA sequence similarities to SM1502(97.6–97.7 %) and XIN-1(96.5–96.6 %). Moreover, strains SaA2.12 and LaA7.5 showed 99.73 % 16S rRNA sequence similarity to each other. Major fatty acids, respiratory quinones and polar lipids detected in these isolates were iso-C, menaquinone-6 and phosphatidylethanolamine, respectively. Strains SaA2.12 and LaA7.5 showed significant unique characteristics between them as well as between the closest phylogenetic members. The highest digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between SaA2.12 and its closest neighbours were 25.3 and 82.8 %, respectively; whereas these values (highest) between LaA7.5 and its closest members were 25.2 and 82.8 %, respectively. The dDDH and ANI values between strains SaA2.12 and LaA7.5 were calculated as 75.9 and 97.2 %, respectively. Therefore, based on polyphasic data, we propose that strain SaA2.12 represents a novel species with the name sp. nov., with the type strain SaA2.12 (=KCTC 72220=MCCC 1K03618) and strain LaA7.5 as a subspecies within novel with the name subsp. subsp. nov., with the type strain LaA7.5 (=KCTC 72806=MCCC 1K04372). These propositions automatically create subsp. subsp. nov. with SaA2.12 (=KCTC 72220=MCCC 1K03618) as the type strain.

Funding
This study was supported by the:
  • Natural Science Foundation of Zhejiang Province (Award LY18D060003)
    • Principle Award Recipient: Pin-Mei Wang
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2020-06-22
2021-10-25
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