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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 7

sp. nov. isolated from mangrove sediment Free

Abstract

A Gram-stain-negative, short-rod-shaped and pink-pigmented bacterial strain (HB172049) was isolated from mangrove sediment. Cells grew at 10–45 °C (optimum, 30 °C), pH 6.0–9.0 (optimum, pH 7.0) and with 0.5–9.0 % (w/v) NaCl (optimum, 2–5 %). Analysis of the 16S rRNA gene sequence revealed that the isolate had highest sequence similarities to DSM 100162 (96.5 %) and X14-1 (96.5 %). The values of average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization between the isolate and its close neighbours were, respectively, less than 80.1, 81.7 and 23.2 %. Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-7 and the predominant cellular fatty acids were summed feature 4 and iso-C (42.2 and 24.6 %, respectively). The major polar lipids consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid, one unidentified phospholipid, one unidentified aminophospholipid and two unidentified polar lipids. The genomic DNA G+C content was 52.6 mol%. Based on polyphasic taxonomic characterization, it is proposed that strain HB172049 belongs to the genus and represents a novel species, for which the name sp. nov. is proposed. The type strain is HB172049 (=CGMCC 1.16729=JCM 33333).

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  • Accepted:
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Keyword(s): 16S rRNA gene , mangrove sediment , polyphasic taxonomy and Pontibacter mangrovi sp. nov.
Funding
This study was supported by the:
  • Central Public-interest Scientific Institution Basal Research Fund of CATAS from Chinese Government (Award 1630052016011, 19CXTD-32)
    • Principle Award Recipient: Yong-hua Hu
  • Financial Fund of the Ministry of Agriculture and Rural Affairs of China (Award NFZX2018))
    • Principle Award Recipient: Hui-qin Huang
© 2020 The Authors
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2020-06-22
2024-04-19
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Pontibacter mangrovi sp. nov., isolated from mangrove sediment
Int J Syst Evol Microbiol 70, 4245 (2020); https://doi.org/10.1099/ijsem.0.004280
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