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Abstract

A Gram-stain-negative, short-rod-shaped and pink-pigmented bacterial strain (HB172049) was isolated from mangrove sediment. Cells grew at 10–45 °C (optimum, 30 °C), pH 6.0–9.0 (optimum, pH 7.0) and with 0.5–9.0 % (w/v) NaCl (optimum, 2–5 %). Analysis of the 16S rRNA gene sequence revealed that the isolate had highest sequence similarities to DSM 100162 (96.5 %) and X14-1 (96.5 %). The values of average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization between the isolate and its close neighbours were, respectively, less than 80.1, 81.7 and 23.2 %. Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-7 and the predominant cellular fatty acids were summed feature 4 and iso-C (42.2 and 24.6 %, respectively). The major polar lipids consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid, one unidentified phospholipid, one unidentified aminophospholipid and two unidentified polar lipids. The genomic DNA G+C content was 52.6 mol%. Based on polyphasic taxonomic characterization, it is proposed that strain HB172049 belongs to the genus and represents a novel species, for which the name sp. nov. is proposed. The type strain is HB172049 (=CGMCC 1.16729=JCM 33333).

Funding
This study was supported by the:
  • Yong-hua Hu , Central Public-interest Scientific Institution Basal Research Fund of CATAS from Chinese Government , (Award 1630052016011, 19CXTD-32)
  • Hui-qin Huang , Financial Fund of the Ministry of Agriculture and Rural Affairs of China , (Award NFZX2018))
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/content/journal/ijsem/10.1099/ijsem.0.004280
2020-06-22
2020-10-20
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