1887

Abstract

A Gram-stain-negative, long-rod-shaped and facultative aerobic bacterium, designated HB-1, was isolated from a round hay bale at the Kansas State University Beef Stocker Unit. The results of phylogenetic analysis of 16S rRNA gene sequences indicated that strain HB-1 clustered within the genus and its closest relatives were A1-9 (98.0 %), YJ-T1-11 (98.0 %), JS43 (97.8 %), DSM 3857 (97.5 %) and Orc-4 (96.9 %). Additional phylogenomic analysis also indicated that strain HB-1 belongs to the genus . The draft genome of strain HB-1 had a total length of 4.23 Mbp and contained 4071 protein-coding genes. The average nucleotide identity values between the genomes of strain HB-1 and the three most-related type strains ranged from 77.5 to 78.1 %. The DNA G+C content of strain HB-1 was 63.7 mol%. The novel strain grew at 10–37 °C, pH 5–10 and with 0–2 % NaCl. Oxidase and catalase activities were positive. Cells were 0.3–0.4 µm wide, 3.0–7.0 µm long and usually found in pairs or chains of cells. The major respiratory quinone of strain HB-1 was Q-10 (90 %), with a minor amount of Q-9 (10 %). The major fatty acids were C ω7 (54.6 %) and C (18.2 %). On the basis of phenotypic, phylogenetic and chemotaxonomic data, strain HB-1 (=DSM 109828=ATCC TSD-211) is considered to represent a novel species of the genus , for which the name sp. nov. is proposed.

Funding
This study was supported by the:
  • JEONGDAE IM , National Science Foundation , (Award OIA-1656006)
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2020-06-18
2020-11-25
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