1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 7

sp. nov. and f.a., sp. nov., two novel yeast species associated with insects and rotting wood Free

Abstract

Five yeast strains were isolated from the gut of the groundbeetle and rotting wood, which were collected from two different localities in China. These strains were identified as representing two novel species of the genus through comparison of sequences in the D1/D2 domains of the LSU rRNA gene and other taxonomic characteristics. sp. nov. produces two to three spherical ascospores per ascus, and is most closely related to the type strains of , , , and an undescribed species represented by strain BG02-7-20-006A-3-1. sp. nov. differed from these strains by 3.6–8.4 % divergence (21–46 substitutions and 0–4 gaps) in the D1/D2 sequences. f.a., sp. nov. is closely related to , and but the formation of ascospores was not observed on various sporulation media, and it differed from its relatives by 6.2–8.5 % divergence (34–43 substitutions and 2–6 gaps) in the D1/D2 sequences. The holotype of sp. nov. is NYNU 1581 and the holotype of f.a., sp. nov. is NYNU 181030.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): anamorphic yeast species , Blastobotrys baotianmanensis , Blastobotrys xishuangbannaensis , groundbeetle and rotten wood
© 2020 The Authors
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004275
2020-06-26
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/70/7/4217.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.004275&mimeType=html&fmt=ahah

References

  1. Klopotek A. Blastobotrys nivea gen.nov., sp.nov. Arch Mikrobiol 1967; 58:92–96 [View Article]
     [Google Scholar]
  2. Kurtzman CP, Robnett CJ. Molecular relationships among hyphal ascomycetous yeasts and yeastlike taxa. Can J Bot 1995; 73:824–830 [View Article]
     [Google Scholar]
  3. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998; 73:331–371 [View Article]
     [Google Scholar]
  4. Kurtzman CP, Robnett CJ. Multigene phylogenetic analysis of the Trichomonascus, Wickerhamiella and Zygoascus yeast clades, and the proposal of Sugiyamaella gen. nov. and 14 new species combinations. FEMS Yeast Res 2007; 7:141–151 [View Article]
     [Google Scholar]
  5. Kurtzman CP, Fell JW, Boekhout T. Blastobotrys von Klopotek. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study 2., 5th ed. Amsterdam: Elsevier; 2011 pp 959–977
     [Google Scholar]
  6. Fell JW, Statzell AC. Sympodiomyces gen. n., a yeast-like organism from southern marine waters. Antonie van Leeuwenhoek 1971; 37:359–367 [View Article]
     [Google Scholar]
  7. Van der Walt JP, Smith MT, Yamada Y. Arxula gen. nov. (Candidaceae), a new anamorphic, arthroconidial yeast genus. Antonie van Leeuwenhoek 1990; 57:59–61 [View Article][PubMed]
     [Google Scholar]
  8. Barretto DA, Avchar R, Carvalho C, Sampaio JP, Vootla SK et al. Blastobotrys bombycis sp. nov., a D-xylose-fermenting yeast isolated from the gut of the silkworm larva Bombyx mori . Int J Syst Evol Microbiol 2018; 68:2638–2643 [View Article]
     [Google Scholar]
  9. Nouri H, Moghimi H, Geranpayeh Vaghei M, Nasr S. Blastobotrys persicus sp. nov., an ascomycetous yeast species isolated from cave soil. Antonie van Leeuwenhoek 2018; 111:517–524 [View Article]
     [Google Scholar]
  10. Hui F-L, Chen L, Chu X-Y, Niu Q-H, Ke T. Wickerhamomyces mori sp. nov., an anamorphic yeast species found in the guts of wood-boring insect larvae. Int J Syst Evol Microbiol 2013; 63:1174–1178 [View Article]
     [Google Scholar]
  11. Hui F-L, Chen L, Li Z-H, Niu Q-H, Ke T. Metschnikowia henanensis sp. nov., a new anamorphic yeast species isolated from rotten wood in China. Antonie van Leeuwenhoek 2013; 103:899–904 [View Article]
     [Google Scholar]
  12. Zheng J, Liu K-F, Liu X-J, Zhang L, Hui F-L et al. Deakozyma yunnanensis sp. nov., a novel yeast species isolated from rotten wood. Int J Syst Evol Microbiol 2017; 67:2436–2439 [View Article]
     [Google Scholar]
  13. Suh SO, Blackwell M. Three new beetle-associated yeast species in the Pichia guilliermondii clade. FEMS Yeast Res 2004; 5:87–95
     [Google Scholar]
  14. Nguyen NH, Suh S-O, Blackwell M. Five novel Candida species in insect-associated yeast clades isolated from Neuroptera and other insects. Mycologia 2007; 99:842–858 [View Article]
     [Google Scholar]
  15. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study 1, 5th edn. Amsterdam: Elsevier; 2011 pp pp. 87–110
     [Google Scholar]
  16. Yarrow D. Methods for the isolation, maintenance and identification of yeasts. In Kurtzman CP, Fell JW. (editors) The Yeasts, a Taxonomic Study, 4th ed. Amsterdam: Elsevier; 1998 pp 77–100
     [Google Scholar]
  17. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: a Guide to Methods and Applications New York: Academic Press; 1990 pp 315–322
     [Google Scholar]
  18. Altschul S et al. Gapped blast and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997; 25:3389–3402 [View Article]
     [Google Scholar]
  19. Thompson J, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DJ. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article]
     [Google Scholar]
  20. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article]
     [Google Scholar]
  21. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article]
     [Google Scholar]
  22. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
     [Google Scholar]
  23. Vu D, Groenewald M, Szöke S, Cardinali G, Eberhardt U et al. DNA barcoding analysis of more than 9000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation. Stud Mycol 2016; 85:91–105 [View Article]
     [Google Scholar]
  24. P. Kurtzman C. Trichomonascus petasosporus sp. nov. and Sympodiomyces indianaensis sp. nov., two new members of the Saccharomycetales. Antonie van Leeuwenhoek 2004; 85:297–304 [View Article]
     [Google Scholar]
  25. MTh S, de Hoog GS, Stephanoascus M. Th. Smith, van der Walt & E. Johannsen. In Kurtzman CP, Fell JW. (editors) The Yeasts, a Taxonomic Study, 4th ed. Amsterdam: Elsevier; 1998 pp 400–403
     [Google Scholar]
  26. Smith MT, de Hoog GS, Statzell-Tallman A, Kurtzman CP. .Blastobotrys von Klopotek. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study, 5th ed. Amsterdam: Elsevier; 1967
     [Google Scholar]
  27. Crous PW, Wingfield MJ, Richardson DM, Leroux JJ, Strasberg D et al. Fungal planet description sheets: 400–468. Pers - Int Mycol J 2016; 36:316–458 [View Article]
     [Google Scholar]
  28. Vega FE, Dowd PF. The role of yeasts as insect endosymbionts. In: Insect–Fungal Associations Ecology and Evolution 2005 pp 211–243
     [Google Scholar]
  29. Suh S-O, McHugh JV, Pollock DD, Blackwell M. The beetle gut: a hyperdiverse source of novel yeasts. Mycol Res 2005; 109:261–265 [View Article]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004275
Loading
Blastobotrys baotianmanensis sp. nov. and Blastobotrys xishuangbannaensis f.a., sp. nov., two novel yeast species associated with insects and rotting wood
Int J Syst Evol Microbiol 70, 4217 (2020); https://doi.org/10.1099/ijsem.0.004275
/content/journal/ijsem/10.1099/ijsem.0.004275
/content/journal/ijsem/10.1099/ijsem.0.004275
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error