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Volume 70, Issue 7

sp. nov., isolated from deep sea Free

Abstract

A Gram-stain-negative, facultative anaerobic, red-pigmented, rod-shaped and non-motile strain (RC1_OXG_1F) was isolated from deep sea water of the Indian Ocean. It was able to grow at pH 5–8 (optimum, pH 7), at 5–37 °C (optimum, 30 °C), and at salinity (% NaCl, w/v) of 0.5–5 % (optimum, 1–2 %). Catalase- and oxidase-positive. It had highest 16S rRNA gene similarity (96.7 %) to ‘’ DH1, followed by JAM-BA0302 (96.6 %), CGMCC 1.9156 (96.2 %), HG677 (96.0 %) and species (less than 92.3 %) of other genera. It belongs to the genus within the phylum , based on the phylogenetic analysis. The results of digital DNA–DNA hybridization and average nucleotide identity analyses indicated that the strain belonged to a novel species. Its genome size is 5,250, 885 bp, with DNA G+C content of 40.5 mol%. Genome analysis revealed that the strain possessed many genes involved in polysaccharide degradation, especially hemicellulose degradation, indicating that the strain could maintain its normal metabolism by using recalcitrant organic matter in the oligotrophic deep sea environment. Its principal fatty acids were anteiso-C and iso-C and the major polar lipids were phosphatidylethanolamine, two unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids. The isoprenoid quinone was MK-7. Based on the phenotypic, chemotaxonomic and genotypic data, the strain represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is RC1_OXG_1F (=MCCC 1A13858=KCTC 62805).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): deep sea , polysaccharide degradation , Sunxiuqinia and taxonomy
Funding
This study was supported by the:
  • National Infrastructure of Microbial Resources of China (Award NIMR-2019-9)
    • Principle Award Recipient: Zongze Shao
  • COMRA program (Award DY135-B2-01)
    • Principle Award Recipient: Zongze Shao
  • NSFC Hydrosphere Project (Award 91851203)
    • Principle Award Recipient: Zongze Shao
© 2020 The Authors
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2020-06-15
2024-04-16
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Sunxiuqinia indica sp. nov., isolated from deep sea
Int J Syst Evol Microbiol 70, 4186 (2020); https://doi.org/10.1099/ijsem.0.004273
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