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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 7

sp. nov., isolated from hospital sewage Free

Abstract

A novel Gram-negative, obligate aerobic, mobile, rod-shaped and non-spore-forming bacterial strain, WCHP16, was isolated from the wastewater treatment plant at West China Hospital in Chengdu, PR China. It was characterized using a polyphasic approach. Analysis of its 16S rRNA gene sequence showed that strain WCHP16 belonged to the genus with the highest similarity to JJ3 (99.34 %), VM14 (99.0 %), NBRC 103162 (99.0 %) and RYU5 (99.0 %). Phylogenomic analysis based on 107 core gene sequences demonstrated that WCHP16 was a member of the group but was distant from all closely related species. Whole-genome comparisons, using average nucleotide identity based on (ANIb) and DNA–DNA hybridization (DDH), confirmed low genome relatedness to all the known species (below the recommended thresholds of 95 % [ANIb] and 70 % [DDH] for species delineation). Phenotypic characterization tests showed that the utilization of phenylacetic acid and capric acid, but not -arabitol, and inability to produce fluorescent (King B medium) in combination could distinguish this strain from other related species of the genus . Therefore, based on genetic and phenotypic evidence, it is clear that strain WCHP16 represents a novel species, for which the name sp. nov. is proposed. The type strain is WCHP16 (GDMCC1.1215=CCTCC AB 2017103=KCTC 52991).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , core genome phylogeny , hospital sewage , Pseudomonas defluvii and whole genome sequence
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 81772233, 81661130159 and 81861138055)
    • Principle Award Recipient: Zhiyong Zong
© 2020 The Authors
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2020-06-18
2025-01-19
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Pseudomonas defluvii sp. nov., isolated from hospital sewage
Int J Syst Evol Microbiol 70, 4199 (2020); https://doi.org/10.1099/ijsem.0.004272
/content/journal/ijsem/10.1099/ijsem.0.004272
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