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Volume 70, Issue 7

sp. nov., a new antifungal species isolated from potato () Free

Abstract

A novel bacterial strain, S40, with strong antifungal activity was isolated from the rhizosphere of green potato collected from Zealand, Denmark. Polyphasic analysis with a combined phenotypic, phylogenetic and genomic approach was used to characterize S40. Phylogenetic analysis based on the 16S rRNA gene and MLSA (concatenated , , and sequences) showed that strain S40 was affiliated with the genus and with PRI-2C as the closest related strain [average nucleotide identity (ANI), 99.26 %; DNA–DNA hybridization (dDDH), 99.20%]. However, whole genome sequence analyses revealed that S40 and PRI-2C genomes displayed lower similarities when compared to all other strains (ANI ≤94.34 %; dDDH ≤57.6 % relatedness). The DNA G+C content of strain S40 was determined to be 55.9 mol%. Cells of the strain were Gram-negative, rod-shaped, facultative anaerobic and displayed growth at 10–37 °C (optimum, 25–30 °C) and at pH 6–9 (optimum, pH 6–7). Major fatty acids were C (27.9 %), summed feature (C 6/C ω7; 18.0 %) and C cyclo (15.1 %). The respiratory quinone was determined to be Q8 (94 %) and MK8 (95 %) and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The results of phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S40 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S40 (=LMG 31467=NCIMB 15235). In addition, we propose that PRI-2C is reclassified and transferred to the species as PRI-2C.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ANI , antimicrobial activity , dDDH , potato , Serratia and whole genome analysis
Funding
This study was supported by the:
  • Innovationsfonden (Award 5158-00001A)
    • Principle Award Recipient: Not Applicable
© 2020 The Authors
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2020-06-17
2024-04-18
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Serratia inhibens sp. nov., a new antifungal species isolated from potato (Solanum tuberosum)
Int J Syst Evol Microbiol 70, 4204 (2020); https://doi.org/10.1099/ijsem.0.004270
/content/journal/ijsem/10.1099/ijsem.0.004270
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