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Abstract

A Gram-stain-negative, yellow-pigmented, aerobic, non-spore-forming, motile with a single polar flagellum and rod-shaped bacterium, Ji-3-8, was isolated from a soil sample taken from Jiri Mountain, Republic of Korea. Comparative 16S rRNA gene sequence studies showed the isolate had clear affiliation with and the closest relatedness to KCTC 52515, ATCC 15253, ATCC 21756, THG-AG3.4, RHGG3 and CB51 showing 99.1, 98.9, 97.7, 97.6, 97.5 and 97.4 % 16S rRNA gene sequence similarity, respectively, and 94.7–96.5 % to the remaining species of genus . The predominant ubiquinone was Q-10 and the major fatty acids were C 7 11-methyl, C, summed feature 8 (C 6 and/or C 7) and summed feature 3 (C ω and/or C 7). The major polar lipids were found to be phosphatidylglycerol, two unidentified phosphoglycolipid and two unidentified glycolipids. The G+C content of the genomic DNA of strain Ji-3-8 was 68.1 mol%. Average nucleotide identity and digital DNA–DNA hybridization values of strain Ji-3-8 with KCTC 52515, ATCC 15253, ATCC 21756, RHGG3 and were 79.7–87.7% and 23.0–34.3%, respectively. Based on the polyphasic evidence, it is proposed that strain Ji-3-8 forms a novel species in the genus , for which the name sp. nov. is proposed. The type strain is Ji-3-8 (=CCTCC AB 2019389=KCTC 72990).

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2020-06-11
2020-10-20
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