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Volume 70, Issue 6

sp. nov., a halophilic bacterium isolated from a polychaete tube in a hydrothermal field Free

Abstract

A moderately halophilic bacterium, designated strain KX18D6, was isolated from the tube of the polychaete collected from a hydrothermal field located in the Okinawa Trough. Strain KX18D6 was Gram-stain-negative, rod-shaped, facultatively anaerobic, motile, oxidase- and catalase-positive, and grew optimally at 30–35 °C, pH 7.0 and in the presence of 3–5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KX18D6 grouped with the members of the genus , including BH030046 (97.7 % sequence similarity), DPSR-4 (97.2 %) and N102 (96.4 %). Genome sequencing of strain KX18D6 revealed a genome size of 4.16 Mb and a DNA G+C content of 47.3 mol%. Genomic average nucleotide identity (orthoANI) values of strain KX18D6 with DSM 16280, KCTC 23464 and N102 were 76.2, 73.1 and 73.2 %, respectively, while the DNA–DNA hybridization (GGDC) values for strain KX18D6 with these strains were 25.3, 17.7 and 18.0 %, respectively. The major fatty acids were summed feature 3 (C ω7/C ω6), C and summed feature 8 (C ω7/C ω6). The predominant respiratory quinone was ubiquinone 8, and the predominant polar lipids were phosphatidylethanolamine and phosphatidylglycerol. On the basis of comparative analysis of phylogenetic, phylogenomic, phenotypic and chemotaxonomic characteristics, strain KX18D6 (=KCTC 72464=MCCC 1K03884) is clearly distinguishable from the type strains of species of the genus and is considered to represent a novel species of the genus , for which the name sp. nov. is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): chemotaxonomic characterization , genome , hydrothermal vent , Salinimonas and type strain
Funding
This study was supported by the:
  • the Open Research Project of National Major Science & Technology Infrastructure (Award NMSTI-KEXUE2017K01)
    • Principle Award Recipient: Huan Zhang
  • National Key R&D Program of China (Award 2018YFC0310800)
    • Principle Award Recipient: Huan Zhang
© 2020 The Authors
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2020-06-04
2024-04-25
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Salinimonas iocasae sp. nov., a halophilic bacterium isolated from a polychaete tube in a hydrothermal field
Int J Syst Evol Microbiol 70, 3899 (2020); https://doi.org/10.1099/ijsem.0.004258
/content/journal/ijsem/10.1099/ijsem.0.004258
/content/journal/ijsem/10.1099/ijsem.0.004258
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