@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004246, author = "Baltaci, Mustafa Ozkan and Ay, Hilal and Akbulut, Sumeyya and Adiguzel, Gulsah and Albayrak, Seyda and Omeroglu, Mehmet Akif and Ozkan, Hakan and Taskin, Mesut and Adiguzel, Ahmet", title = "Bacillus pasinlerensis sp. nov., a thermophilic bacterium isolated from a hot spring in Turkey", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "6", pages = "3865-3871", doi = "https://doi.org/10.1099/ijsem.0.004246", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004246", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "new species", keywords = "Bacillus", keywords = "polyphasic taxonomy", keywords = "hot spring", keywords = "thermophilic", abstract = "A Gram-reaction-positive, endospore-forming bacterium, designated strain P1T, was isolated from water samples collected from Pasinler Hot Spring and characterized using a polyphasic approach to clarify its taxonomic position. Strain P1T was found to have chemotaxonomic and morphological characteristics consistent with its classification in the genus Bacillus . The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences. Strain P1T could grow optimally at 55 °C and in the presence of 2 % NaCl. The organism was found to contain meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The predominant menaquinone was determined to be MK-7. The major cellular fatty acids were identified as iso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0. Based upon the consensus of phenotypic and phylogenetic analyses, strain P1T represents a novel species of the genus Bacillus , for which the name Bacillus pasinlerensis sp. nov. is proposed. The type strain is P1T (=DSM 107529T=CECT 9885T=NCCB 100674T).", }