1887

Abstract

A Gram-reaction-positive, endospore-forming bacterium, designated strain P1, was isolated from water samples collected from Pasinler Hot Spring and characterized using a polyphasic approach to clarify its taxonomic position. Strain P1 was found to have chemotaxonomic and morphological characteristics consistent with its classification in the genus . The strain shared the highest 16S rRNA gene sequence identity values with R-6488 (97.6 %) and MO-04 (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences. Strain P1 could grow optimally at 55 °C and in the presence of 2 % NaCl. The organism was found to contain meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The predominant menaquinone was determined to be MK-7. The major cellular fatty acids were identified as iso-C, iso-C and anteiso-C. Based upon the consensus of phenotypic and phylogenetic analyses, strain P1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is P1 (=DSM 107529=CECT 9885=NCCB 100674).

Funding
This study was supported by the:
  • Atatürk Üniversitesi (Award FBA-2019-7225)
    • Principle Award Recipient: Ahmet ADIGUZEL
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2020-06-04
2021-10-16
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