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Abstract

A novel marine bacterium, designated strain 216LB-ZA1-12, was isolated from a aquaculture seawater sample. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 216LB-ZA1-12 belonged to the genus , with the highest sequence similarity to MKT133 (97.7 %), followed by GH1-24 (97.0 %), CAU 1294 (96.6 %) and LMG 23452 (96.1 %). The average nucleotide identity and the DNA–DNA hybridization estimate values between strain 216LB-ZA1-12 and four close type strains were between 69.2–71.3% and 16.7–17.8 %, respectively. The bacterium was Gram-stain-negative, facultatively anaerobic, oxidase and catalase positive, oval- to rod-shaped, and motile. Growth was observed at pH 7–9, salinities of 1–15% and temperatures of 4–42 °C. The G+C content of the chromosomal DNA was 48.50 mol%. The major respiratory quinone was determined to be Q-10. The principal fatty acids were summed feature 8 (C ωω) and C. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and aminophospholipid. The combined genotypic and phenotypic data show that strain 216LB-ZA1-12 represents a novel species within the genus , for which the name sp. nov. is proposed, with the type strain 216LB-ZA1-12 (=MCCC 1A14570=KCTC 72144).

Funding
This study was supported by the:
  • Zongze Shao , Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province (CN) , (Award NIMR-2020-9)
  • Zongze Shao , Ministère du Développement Économique, de l’Innovation et de l’Exportation (CA) , (Award 16PZP001SF16)
  • Zongze Shao , National Natural Science Foundation of China , (Award No.41676149)
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2020-05-29
2020-10-20
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