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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 6

sp. nov. and sp. nov., isolated from a saline lake Free

Abstract

Two novel strains, designated SYSU L10167 and SYSU L10180, were isolated from sediment sampled at Dabancheng saline lake in Xinjiang, PR China. A polyphasic approach was used to clarify the taxonomic positions of the two strains. Cells of the isolates were curved ring-like, horseshoe-shaped or rod-shaped, non-motile and non-spore-forming. Cells were Gram-stain-negative, aerobic, heterotrophic and rose-pigmented. The phylogenetic trees based on 16S rRNA gene sequences showed that strains SYSU L10167 and SYSU L10180 formed a distinct lineage within the genus . Strains SYSU L10167 and SYSU L10180 showed highest similarities to KCTC 23150 (98.0 and 97.4%, respectively). Results of genomic analyses (including average nucleotide identity, digital DNA–DNA hybridization and the marker gene tree) and pan-genome analysis further confirmed that strains SYSU L10167 and SYSU L10180 were separate from each other and other species of the genus . The draft genomes of the isolates had sizes of 5.5–5.7 Mb and reflected their major physiological capabilities. Based on phenotypic, physiological, chemotaxonomic and genotypic characterization, we propose that the isolates represent two novel species, for which the names sp. nov. and sp. nov. are proposed. The type strains of the species are SYSU L10167 (=KCTC 72390=CGMCC 1.17521) and SYSU L10180 (=KCTC 72391=CGMCC 1.17278).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Cyclobacterium , Cyclobacterium roseum sp. nov , Cyclobacterium salsum sp. nov. , saline lake and taxogenomics
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 91751206)
    • Principle Award Recipient: Yong-Hong Liu
  • Department of Education, Xinjiang Uygur Autonomous Region (CN) (Award 2017E01031)
    • Principle Award Recipient: Wen-Jun Li
  • National Key R&D Program of China (Award 2017FY100300)
    • Principle Award Recipient: Wen-Jun Li
© 2020 Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, China
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2020-05-28
2023-03-21
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Cyclobacterium salsum sp. nov. and Cyclobacterium roseum sp. nov., isolated from a saline lake
Int J Syst Evol Microbiol 70, 3785 (2020); https://doi.org/10.1099/ijsem.0.004237
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