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Volume 70, Issue 6

sp. nov., a moderate halophile isolated from saline soil in Northwest China Free

Abstract

A moderately halophilic strain, designated SCU50, was recovered from a saline soil sample and characterized by a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain SCU50 belonged to the genus and was most closely related to TP2-8 (98.1 % similarity) and XH-63 (97.7 %). Genomic average nucleotide identity and digital DNA–DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized species. The genome size was about 5.09 Mbp and the DNA G+C content was 36.7 mol%. The strain grew optimally at 10–15 % (w/v) NaCl, pH 6.5–7.5 and 25–30 °C. It contained anteiso-C, iso-C and anteiso-C as the dominant fatty acids and menaquinone-7 as the major respiratory quinone. The polar lipid profile was examined and found to comprise diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and one unidentified lipid. The cell-wall peptidoglycan type was A1γ based on -diaminopimelic acid. Combining the data from phenotypic, chemotaxonomic, genomic and phylogenetic characterization, it was concluded that strain SCU50 should be assigned as representing a novel species within the genus . Thus, a novel taxon named sp. nov. was first established, with SCU50 (=CGMCC 1.17336=KCTC 43107) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Gracilibacillus salitolerans sp. nov. , moderate halophile , polyphasic taxonomy and saline soil
Funding
This study was supported by the:
  • Opening Project of Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education (Award 20826041C4159)
    • Principle Award Recipient: Yongqiang Tian
  • National Key Research and Development Program of China (Award 2018YFC1802201)
    • Principle Award Recipient: Yongqiang Tian
© 2020 The Authors
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2020-05-15
2024-04-19
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Gracilibacillus salitolerans sp. nov., a moderate halophile isolated from saline soil in Northwest China
Int J Syst Evol Microbiol 70, 3701 (2020); https://doi.org/10.1099/ijsem.0.004224
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