@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004222, author = "Verma, Ashish and Pal, Yash and Kumar, Pravin and Krishnamurthi, Srinivasan", title = "Halocatena pleomorpha gen. nov. sp. nov., an extremely halophilic archaeon of family Halobacteriaceae isolated from saltpan soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "6", pages = "3693-3700", doi = "https://doi.org/10.1099/ijsem.0.004222", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004222", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "16S rRNA", keywords = "SPP-AMP-1T", keywords = "Halobacteriaceae", abstract = "A novel archaeal strain designated as SPP-AMP-1T was isolated from saltpan soil, using the serial dilution method on a halophilic archaeal medium supplemented with ampicillin. Cells were both rod-shaped and pleomorphic in nature, non-motile, unable to produce acid from a variety of sugars or grow anaerobically with different substrates (l-arginine) and electron acceptors (DMSO, nitrate). Optimal growth was observed at 42 °C, 3.4–4.2 M NaCl and pH 7.2. Cells did not lyse in distilled water and grew in the absence of Mg2+ ions. Phylogenetic analysis based on the sequences of 16S rRNA gene, amino acid sequence of β′-subunit of RNA polymerase and 400 conserved proteins retrieved from the whole genome assemblies showed that strain SPP-AMP-1T was distantly related to any existing genera within the family Halobacteriaceae . MK-8 was the only quinone detected. Polar lipid analysis showed a unique combination of diethyl derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, glycosyl-mannosyl-glucosyl diether and sulphated glycosyl-mannosyl-glucosyl diether as the major lipids. The G+C content of genomic DNA is 57.7 mol%. The phenotypic, phylogenetic and genomic data supported the concept of the novel genus status of strain SPP-AMP-1T in the family Halobacteriaceae for which the name Halocatena pleomorpha gen. nov., sp. nov., is proposed; the type strain is SPP-AMP-1T (=JCM 31368T=KCTC 4276T=MTCC 12579T).", }