1887

Abstract

A Gram-stain-negative, rod-shaped (0.2–0.3×1.0-2.4 µm), catalase-positive, oxidase-negative and non-motile bacterium, designated strain RZ26, was isolated from the marine red algae collected from the coast of Weihai, PR China. Growth of strain RZ26 occurred at 15–33 °C (optimum, 25–28 °C), pH 6.0–9.5 (optimum, pH 7.0–7.5) and 0.5–5.0 % (w/v) NaCl (optimum, 2.0–3.0 %). Resuls of phylogenetic analysis based on 16S rRNA gene sequences showed that strain RZ26 was most closely related to DSM 25233 (96.2 % sequence similarity), followed by DSM 18668 (96.1 %) and DSM 25230 (95.4 %). The average nucleotide identity and the average amino acid identity values between strain RZ26 and KCTC 12966, DSM 25233, DSM 25230 and DSM 18668 were 75.6, 76.2, 76.0, 76.7, 64.3, 63.9, 68.6 and 68.0 %, respectively. The digital DNA–DNAhybridization values based on the draft genomes between strain RZ26 and KCTC 12966, DSM 25233 and DSM 25230 were 38.0, 35.1 and 37.1 %, respectively. The major fatty acids in strain RZ26 were iso-C 3-OH, iso-C and C 7/C 6. The major respiratory quinone was MK-6. The dominant polar lipid was phosphatidylethanolamine. The DNA G+C content was 38.0 mol%. Phylogenetic analysis shows strain RZ26 fell within a clade comprising species of the genus . Polyphasic taxonomy indicates that the isolate represents a novel species of the genus , for which the name sp. nov. is proposed, with type strain RZ26 (=KCTC 62992=MCCC 1H00362).

Funding
This study was supported by the:
  • Zong-Jun Du , the Science and Technology Basic Resources Investigation Program of China , (Award 2017FY100300)
  • Zong-Jun Du , the National Natural Science Foundation of China , (Award 31770002)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004220
2020-05-15
2020-06-02
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/10.1099/ijsem.0.004220/ijsem004220.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.004220&mimeType=html&fmt=ahah

References

  1. Mu DS, Liang QY, Wang XM, Lu DC, Shi MJ et al. Metatranscriptomic and comparative genomic insights into resuscitation mechanisms during enrichment culturing. Microbiome 2018; 6:1–15
    [Google Scholar]
  2. Kirchman DL. The ecology of Cytophaga-Flavobacteria in aquatic environments. FEMS Microbiol Ecol 2002; 39:91–100 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  3. Bernardet J-F, Nakagawa Y, Holmes B. Subcommittee On the Taxonomy of Flavobacterium and Cytophaga-Like Bacteria of the International Committee On Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  4. Nedashkovskaya OI, Kim SB, Han SK, Lysenko AM, Rohde M et al. Maribacter gen. nov., a new member of the family Flavobacteriaceae, isolated from marine habitats, containing the species Maribacter sedimenticola sp. nov., Maribacter aquivirus sp. nov., Maribacter orientalis sp. nov. and Maribacter ulvicola sp. nov. Int J Syst Evol Microbiol 2004; 54:1017–1023 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  5. Nedashkovskaya OI, Kim SB, Mikhailov VV. Maribacter stanieri sp. nov., a marine bacterium of the family Flavobacteriaceae. Int J Syst Evol Microbiol 2010; 60:214–218 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  6. Barbeyron T, Carpentier F, L'haridon S, Schüler M, Michel G et al. Description of Maribacter forsetii sp. nov., a marine Flavobacteriaceae isolated from North Sea water, and emended description of the genus Maribacter. Int J Syst Evol Microbiol 2008; 58:790–797 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  7. Lo N, Jin HM, Jeon CO. Maribacter aestuarii sp. nov., isolated from tidal flat sediment, and an emended description of the genus Maribacter. Int J Syst Evol Microbiol 2013; 63:3409–3414 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  8. Hu J, Yang Q-Q, Ren Y, Zhang W-W, Zheng G et al. Maribacter thermophilus sp. nov., isolated from an algal bloom in an intertidal zone, and emended description of the genus Maribacter. Int J Syst Evol Microbiol 2015; 65:36–41 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  9. Weerawongwiwat V, Kang H, Jung MY, Kim W. Maribacter chungangensis sp. nov., isolated from a green seaweed, and emended descriptions of the genus Maribacter and Maribacter arcticus. Int J Syst Evol Microbiol 2013; 63:2553–2558 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  10. Jackson SA, Kennedy J, Morrissey JP, O'Gara F, Dobson ADW. Maribacter spongiicola sp. nov. and Maribacter vaceletii sp. nov., isolated from marine sponges, and emended description of the genus Maribacter. Int J Syst Evol Microbiol 2015; 65:2097–2103 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  11. Yoon J-H, Kang S-J, Lee S-Y, Lee C-H, Oh T-K. Maribacter dokdonensis sp. nov., isolated from sea water off a Korean island, Dokdo. Int J Syst Evol Microbiol 2005; 55:2051–2055 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  12. Nedashkovskaya OI, Vancanneyt M, De Vos P, Kim SB, Lee MS et al. Maribacter polysiphoniae sp. nov., isolated from a red alga. Int J Syst Evol Microbiol 2007; 57:2840–2843 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  13. Zhang GI, Hwang CY, Kang S-H, Cho BC. Maribacter antarcticus sp. nov., a psychrophilic bacterium isolated from a culture of the Antarctic green alga Pyramimonas gelidicola. Int J Syst Evol Microbiol 2009; 59:1455–1459 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  14. Cho KH, Hong SG, Cho HH, Lee YK, Chun J et al. Maribacter arcticus sp. nov., isolated from Arctic marine sediment. Int J Syst Evol Microbiol 2008; 58:1300–1303 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  15. Kim KH, Jin HM, Jeong HI, Jeon CO. Maribacter lutimaris sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 2016; 66:1773–1778 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  16. Jung Y-T, Lee J-S, Yoon J-H. Maribacter caenipelagi sp. nov., a member of the Flavobacteriaceae isolated from a tidal flat sediment of the Yellow Sea in Korea. Antonie van Leeuwenhoek 2014; 106:733–742 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  17. Liu Q-Q, Wang Y, Li J, Du Z-J, Chen G-J. Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 2014; 64:2204–2209 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  18. Li R, Zhu H, Ruan J, Qian W, Fang X et al. De novo assembly of human genomes with massively parallel short read sequencing. Genome Res 2010; 20:265–272 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  19. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  20. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  21. Arnold G, Kluge JSF. Quantitative Phyletics and the evolution of anurans. Systematic Biology 1969; 18:1–32
    [Google Scholar]
  22. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  23. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50 Pt 5:1861–1868 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  24. Dong XZ, Cai MY. Determination of biochemical characteristics. In Dong XZ, Cai MY. (editors) Manual for the Systematic Identification of General Bacteria Beijing: Science Press; 2001 pp 370–398
    [Google Scholar]
  25. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [CrossRef]
    [Google Scholar]
  26. Xin H, Itoh T, Zhou P, Suzuki K, Kamekura M et al. Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 2000; 50 Pt 3:1297–1303 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  27. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparative systematics. Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: American Society for Microbiology; 2007 pp 330–393
    [Google Scholar]
  28. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 1982; 5:2359–2367 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004220
Loading
/content/journal/ijsem/10.1099/ijsem.0.004220
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error