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Volume 70, Issue 6

sp. nov., a new member of the family isolated from the red alga Free

Abstract

A Gram-stain-negative, rod-shaped (0.2–0.3×1.0-2.4 µm), catalase-positive, oxidase-negative and non-motile bacterium, designated strain RZ26, was isolated from the marine red algae collected from the coast of Weihai, PR China. Growth of strain RZ26 occurred at 15–33 °C (optimum, 25–28 °C), pH 6.0–9.5 (optimum, pH 7.0–7.5) and 0.5–5.0 % (w/v) NaCl (optimum, 2.0–3.0 %). Resuls of phylogenetic analysis based on 16S rRNA gene sequences showed that strain RZ26 was most closely related to DSM 25233 (96.2 % sequence similarity), followed by DSM 18668 (96.1 %) and DSM 25230 (95.4 %). The average nucleotide identity and the average amino acid identity values between strain RZ26 and KCTC 12966, DSM 25233, DSM 25230 and DSM 18668 were 75.6, 76.2, 76.0, 76.7, 64.3, 63.9, 68.6 and 68.0 %, respectively. The digital DNA–DNAhybridization values based on the draft genomes between strain RZ26 and KCTC 12966, DSM 25233 and DSM 25230 were 38.0, 35.1 and 37.1 %, respectively. The major fatty acids in strain RZ26 were iso-C 3-OH, iso-C and C 7/C 6. The major respiratory quinone was MK-6. The dominant polar lipid was phosphatidylethanolamine. The DNA G+C content was 38.0 mol%. Phylogenetic analysis shows strain RZ26 fell within a clade comprising species of the genus . Polyphasic taxonomy indicates that the isolate represents a novel species of the genus , for which the name sp. nov. is proposed, with type strain RZ26 (=KCTC 62992=MCCC 1H00362).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , genome , Maribacter and prokaryotic taxonomy
Funding
This study was supported by the:
  • the Science and Technology Basic Resources Investigation Program of China (Award 2017FY100300)
    • Principle Award Recipient: Zong-Jun Du
  • the National Natural Science Foundation of China (Award 31770002)
    • Principle Award Recipient: Zong-Jun Du
© 2020 The Authors
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2020-05-15
2024-12-03
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Maribacter algarum sp. nov., a new member of the family Flavobacteriaceae isolated from the red alga Gelidium amansii
Int J Syst Evol Microbiol 70, 3679 (2020); https://doi.org/10.1099/ijsem.0.004220
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