f.a., sp. nov., a novel tremellaceous yeast species isolated from soil in a secondary peat swamp forest area Free

Abstract

The strain DMKU-XD44, representing an anamorphic novel yeast species, was isolated from soil collected in a peat swamp forest (PSF) area in Rayong Botanical Garden in eastern Thailand. On the basis of sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, CBS 10309 was the most closely related species. The novel species differed from the type by 2.5 % (14 nucleotide substitutions) in the D1/D2 region of the LSU rRNA gene and by 8.0 % (40 nucleotide substitutions) in the ITS region. The results of a phylogenetic analysis, based on the combined sequences of the ITS region and the D1/D2 region, indicated that DMKU-XD44 represents a member of the clade in the (, , and ) and is phylogenetically distinct from other species of the genus in the clade. Therefore, DMKU-XD44 represents a novel species of the genus . The name f.a., sp. nov. is proposed. The holotype is DMKU-XD44, while the MycoBank number is MB 832816.

Funding
This study was supported by the:
  • Center of Excellence on Biodiversity, Postgraduate Education and Research Development Office (PERDO) (Award BDC-PG1-160001)
    • Principle Award Recipient: Savitree Limtong
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004219
2020-05-14
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/70/6/3673.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.004219&mimeType=html&fmt=ahah

References

  1. Li A-H, Yuan F-X, Groenewald M, Bensch K, Yurkov AM et al. Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: proposal of two new orders, three new families, eight new genera and one hundred and seven new species. Stud Mycol 2020; 96:17–140 [View Article][PubMed]
    [Google Scholar]
  2. de Garcia V, Zalar P, Brizzio S, Gunde-Cimerman N, van Broock M. Cryptococcus species (Tremellales) from glacial biomes in the southern (Patagonia) and northern (Svalbard) hemispheres. FEMS Microbiol Ecol 2012; 82:523–539 [View Article][PubMed]
    [Google Scholar]
  3. Liu X-Z, Wang Q-M, Theelen B, Groenewald M, Bai F-Y et al. Phylogeny of tremellomycetous yeasts and related dimorphic and filamentous basidiomycetes reconstructed from multiple gene sequence analyses. Stud Mycol 2015a; 81:1–26 [View Article][PubMed]
    [Google Scholar]
  4. Liu X-Z, Wang Q-M, Göker M, Groenewald M, Kachalkin AV et al. Towards an integrated phylogenetic classification of the Tremellomycetes. Stud Mycol 2015b; 81:85–147 [View Article][PubMed]
    [Google Scholar]
  5. Sylvester K, Wang Q-M, James B, Mendez R, Hulfachor AB et al. Temperature and host preferences drive the diversification of Saccharomyces and other yeasts: a survey and the discovery of eight new yeast species. FEMS Yeast Res 2015; 15:fov002 [View Article][PubMed]
    [Google Scholar]
  6. Jaiboon K, Lertwattanasakul N, Limtong P, Limtong S. Yeasts from peat in a tropical peat swamp forest in Thailand and their ability to produce ethanol, indole-3-acetic acid and extracellular enzymes. Mycol Prog 2016; 15:755–770 [View Article]
    [Google Scholar]
  7. Posa MRC, Wijedasa LS, Corlett RT. Biodiversity and conservation of tropical peat swamp forests. Bioscience 2011; 61:49–57 [View Article]
    [Google Scholar]
  8. UNDP Malaysia's Peat Swamp Forests Conservation and Sustainable Use Malaysia: UNDP; 2006
    [Google Scholar]
  9. Nuyim T. Peat swamp forests and their importance. In Ritthichuai H, Chaiyakayuth R, Raksasri S, Kaewsooksai S. (editors) Guideline on Peat Swamp Forests Rehabilitation and Planting in Thailand Narathiwat: Pikulthong Royal Development Study Centre National Park; 2003 pp 1–6
    [Google Scholar]
  10. Boonmak C, Khunnamwong P, Limtong S. Yeast communities of primary and secondary peat swamp forests in southern Thailand. Antonie van Leeuwenhoek 2020; 113:55–69 [View Article][PubMed]
    [Google Scholar]
  11. Khunnamwong P, Limtong S. Saturnispora kantuleensis f.a., sp. nov., a novel yeast species isolated from peat in a tropical peat swamp forest in Thailand. Int J Syst Evol Microbiol 2018; 68:1160–1164 [View Article][PubMed]
    [Google Scholar]
  12. Nitiyon S, Khunnamwong P, Lertwattanasakul N, Limtong S. Candida kantuleensis sp. nov., a d-xylose-fermenting yeast species isolated from peat in a tropical peat swamp forest. Int J Syst Evol Microbiol 2018; 68:2313–2318 [View Article][PubMed]
    [Google Scholar]
  13. Kaewwichian R, Khunnamwong P, Jindamorakot S, Lertwattanasakul N, Limtong S. Cryptotrichosporon siamense sp. nov., a ballistoconidium-forming yeast species in Trichosporonales isolated in Thailand. Int J Syst Evol Microbiol 2018; 68:2473–2477 [View Article][PubMed]
    [Google Scholar]
  14. Kaewwichian R, Khunnamwong P, Am-In S, Jindamorakot S, Groenewald M et al. Candida xylosifermentans sp. nov., a d-xylose-fermenting yeast species isolated in Thailand. Int J Syst Evol Microbiol 2019; 69:26742680 [View Article][PubMed]
    [Google Scholar]
  15. Polburee P, Lertwattanasakul N, Limtong P, Groenewald M, Limtong S. Nakazawaea todaengensis f.a., sp. nov., a yeast isolated from a peat swamp forest in Thailand. Int J Syst Evol Microbiol 2017; 67:2377–2382 [View Article][PubMed]
    [Google Scholar]
  16. Limtong S, Yongmanitchai W, Tun MM, Kawasaki H, Seki T. Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand. Int J Syst Evol Microbiol 2007; 57:419–422 [View Article][PubMed]
    [Google Scholar]
  17. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998; 73:331–371 [View Article][PubMed]
    [Google Scholar]
  18. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications New York: Academic Press; 1990 pp 315–322
    [Google Scholar]
  19. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  20. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped blast and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997; 25:3389–3402 [View Article][PubMed]
    [Google Scholar]
  21. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  22. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study, 5th edn. Amsterdam: Elsevier; 2011 pp 87–110
    [Google Scholar]
  23. Kurtzman CP. Description of new yeast species–Is one strain enough?. Bull BISMiS 2010; 1:17–24
    [Google Scholar]
  24. McNeill J, Barrie FR, Buck WR, Demoulin V, Greuter W et al. International Code of Nomenclature for Algae, Fungi, and Plants (Melbourne Code). Regnum Veg 154 Koenigstein, Germany: Gantner Verlag; 2012
    [Google Scholar]
  25. Lachance MA. In defense of yeast sexual life cycles: the forma asexualis–An informal proposal. Yeast Newletter 2012; 6:24–25
    [Google Scholar]
  26. Shin K-S, Oh H-M, Park Y-H, Lee KH, Poo H et al. Cryptococcus mujuensis sp. nov. and Cryptococcus cuniculi sp. nov., basidiomycetous yeasts isolated from wild rabbit faeces. Int J Syst Evol Microbiol 2006; 56:2241–2244 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004219
Loading
/content/journal/ijsem/10.1099/ijsem.0.004219
Loading

Data & Media loading...

Most cited Most Cited RSS feed