1887

Abstract

Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136 and dk3543, were isolated from the faeces of Tibetan gazelle () in the Qinghai–Tibet Plateau of PR China. The cells were aerobic, oxidase-negative and catalase-positive. Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain–heart infusion (BHI) agar with 5 % sheep blood. The cells grew optimally at 28 °C, pH 7.5 and with 1 % (w/v) NaCl on BHI agar supplemented with 5 % sheep blood. Phylogenetic analysis of the 16S rRNA gene sequences revealed that their nearest phylogenetic relative was Ka25 (97.9 % similarity). The results of 16S rRNA gene sequence and phylogenetic/phylogenomic analyses illustrated that Ka25, XZ17, 78 and D287 were their nearest phylogenetic neighbours. The DNA G+C contents of strains dk3136 and dk3543 were 70.3 mol% and 70.4 mol%, respectively. Their genomes exhibit lower than threshold (95–96 %) average nucleotide identity to known species of the genus . -2,6-diaminopimelic acid was the diagnostic diamino acid and MK-8(H) was the predominant respiratory quinone. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The two strains had C 9, -C and C 8 as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars. On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136 and dk3543 represent a novel species in genus , for which the name sp. nov. is proposed. The type strain is dk3136 (=CGMCC 4.7570=JCM 33496=KCTC 49314).

Funding
This study was supported by the:
  • Research Units of Discovery of Unknown Bacteria and Function Chinese Academy of Medical Sciences. (Award 2018RU010)
    • Principle Award Recipient: Jianguo Xu
  • Sanming Project of Medicine in Shenzhen (Award SZSM201811071)
    • Principle Award Recipient: Jianguo Xu
  • National Key R&D Program of China (Award 2018YFC1200102)
    • Principle Award Recipient: Dong Jin
  • the National Science and Technology Major Project of China (Award 2018ZX10712001-018)
    • Principle Award Recipient: Shan Lu
  • National Major Science and Technology Projects of China (CN) (Award 2018ZX10712001-007)
    • Principle Award Recipient: Jing Yang
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2020-05-14
2021-05-14
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