@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004215, author = "Ikeyama, Nao and Toyoda, Atsushi and Morohoshi, Sho and Kunihiro, Tadao and Murakami, Takumi and Mori, Hiroshi and Iino, Takao and Ohkuma, Moriya and Sakamoto, Mitsuo", title = "Amedibacterium intestinale gen. nov., sp. nov., isolated from human faeces, and reclassification of Eubacterium dolichum Moore et al. 1976 (Approved Lists 1980) as Amedibacillus dolichus gen. nov., comb. nov", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "6", pages = "3656-3664", doi = "https://doi.org/10.1099/ijsem.0.004215", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004215", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Absiella", keywords = "human faeces", keywords = "Amedibacterium", keywords = "hsp60", keywords = "Amedibacillus", abstract = "Four strains (9CBEGH2T, 9BBH35, 6BBH38 and 6EGH11) of Gram-stain-positive, obligately anaerobic, rod-shaped bacteria were isolated from faecal samples from healthy Japanese humans. The results of 16S rRNA gene sequence analysis indicated that the four strains represented members of the family Erysipelotrichaceae and formed a monophyletic cluster with ‘ Absiella argi ’ strain N6H1-5 (99.4% sequence similarity) and Eubacterium sp. Marseille-P5640 (99.3 %). Eubacterium dolichum JCM 10413T (94.2 %) and Eubacterium tortuosum ATCC 25548T (93.7 %) were located near this monophyletic cluster. The isolates, 9CBEGH2T, ‘ A. argi ’ JCM 30884 and Eubacterium sp. Marseille-P5640 shared 98.7–99.1% average nucleotide identity (ANI) with each other. Moreover, the in silico DNA–DNA hybridization (DDH) values among three strains were 88.4–90.6%, indicating that these strains represent the same species. Strain 9CBEGH2T showed 21.5–24.1 % in silico DDH values with other related taxa. In addition, the ANI values between strain 9CBEGH2T and other related taxa ranged from 71.2 % to 73.5 %, indicating that this strain should be considered as representing a novel species on the basis of whole-genome relatedness. Therefore, we formally propose a novel name for ‘ A. argi ’ strains identified because the name ‘ A. argi ’ has been effectively, but not validly, published since 2017. On the basis of the collected data, strain 9CBEGH2T represents a novel species of a novel genus, for which the name Amedibacterium intestinale gen. nov., sp. nov. is proposed. The type strain of A. intestinale is 9CBEGH2T (=JCM 33778T=DSM 110575T).", }