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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 6

gen. nov., sp. nov., a member of the family isolated from a human peritoneal tumour and reclassification of as comb. nov. Free

Abstract

A Gram-stain-negative, microaerophilic, non-motile, rod-shaped bacterium strain designated PMP191F, was isolated from a human peritoneal tumour. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the organism formed a lineage within the family that was distinct from members of the genus (95.1–95.2 % sequence similarity) and (94.4 % sequence similarity). The average nucleotide identity values between strain PMP191F and T16R-265 and Gsoil 221 was 68.9 and 62.3% respectively. The only respiratory quinone of strain PMP191F was MK-7 and the major fatty acids were iso-C, iso-C G and summed feature 3 (Cω7 and/or Cω6). The polar lipids consisted of phosphatidylethanolamine and some unidentified amino and glycolipids. The G+C content of strain PMP191F calculated from the genome sequence was 43.4 mol%. Based on phylogenetic, phenotypic and chemotaxonomic evidence, strain PMP191F represents a novel species and genus for which the name gen. nov., sp. nov. is proposed. The type strain is PMP191F (=DSM 104999=ATCC BAA-2857 = CCUG 72691). The phylogenetic analyses also revealed that shared over 98 % sequence similarly to members of the genus . However, the average nucleotide identity value between T16R-265, the type species of the genus and Gsoil 221 was 86.8 %. Therefore, we also propose that be reclassified as comb. nov.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): cancer , Chitinophagaceae , Parapseudoflavitalea , Pseudoflavitalea and Pseudomyxoma peritonei
Funding
This study was supported by the:
  • National Organization for Rare Disorders (Award 17005)
    • Principle Award Recipient: Traci Testerman
  • National Institutes of Health (Award P20GM103641)
    • Principle Award Recipient: Traci Testerman
© 2020 The Authors
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2020-06-05
2025-04-30
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Parapseudoflavitalea muciniphila gen. nov., sp. nov., a member of the family Chitinophagaceae isolated from a human peritoneal tumour and reclassification of Pseudobacter ginsenosidimutans as Pseudoflavitalea ginsenosidimutans comb. nov.
Int J Syst Evol Microbiol 70, 3639 (2020); https://doi.org/10.1099/ijsem.0.004204
/content/journal/ijsem/10.1099/ijsem.0.004204
/content/journal/ijsem/10.1099/ijsem.0.004204
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