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Abstract

A Gram-stain-positive bacterial isolate, designated LMM-1652, was isolated from an intrauterine cytobrush sample originating from a postpartum Holstein Friesian dairy cow. The strain had a rod to coccoid-shape, was catalase-positive and oxidase-negative. 16S rRNA gene sequence similarity analyses revealed that its closest relatives were (97.05 % similarity), (96.83 %) and (96.82 %). Subsequent whole genome analysis showed that the genome-to-genome distance of strain LMM-1652 to its closest relatives was in the range of 23.2–24.8 %, while the average nucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species. Strain LMM-1652 was characterized by a quinone system mainly consisting of MK-9(H) and MK-10(H). The polar lipids profile of the strain consisted mainly of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol-mannoside, as well as one unidentified lipid lacking any functional group. Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found. The cell wall contained -diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan. The fatty acid profile was mainly composed of C ω9, C and C. We propose a novel species of the genus with the name LMM-1652 (=LMG 31163=DSM 108747).

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2020-05-27
2020-10-30
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