1887

Abstract

Bacterial strain TWA-58, isolated from irrigation water in Taiwan, was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain TWA-58 formed a phylogenetic lineage in the genus of the family . Strain TWA-58 was most closely related to NVT with a 96.7 % 16S rRNA gene sequence similarity. Strain TWA-58 showed 75.2 % average nucleotide identity, 70.9 % average amino acid identity and 21.0 % digital DNA–DNA hybridization identity with NVT. Cells were Gram-stain-negative, aerobic, motile, coccoid-shaped and formed transparent colonies. Optimal growth occurred at 25 °C, pH 6, and 0 % NaCl. The major fatty acids of strain TWA-58 were iso-C and anteiso-C. The predominant hydroxy fatty acid was iso-C 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified aminophospholipids. The major isoprenoid quinone was MK-7. Genomic DNA G+C content of strain TWA-58 was 65.3 mol%. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain TWA-58 should be classified in a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TWA-58 (=BCRC 81161=LMG 31019=KCTC 62872).

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2020-05-04
2020-06-02
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