1887

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Volume 70, Issue 6

sp. nov., isolated from mountain soil Free

Abstract

A soil bacterium, designated ZX9611, was isolated from Taihang Mountain in Henan province, PR China. The strain was Gram-stain-negative and strictly aerobic. The cells were motile, rod-shaped and formed light pink-colored colonies. The 16S rRNA gene sequence of ZX9611 shared the highest similarities with those of CCP-7 (97.0%), S5-249 (96.6%) and 382 (95.9%). Phylogenetic analyses based on 16S rRNA gene sequences demonstrated that ZX9611 clustered with CCP-7, S5-249 and 382. The average nucleotide identity (ANI) values between ZX9611 and two type strains ( BCRC 81096 and DSM 27345) were 88.3 and 68.6% respectively. ZX9611 exhibited genome-sequence-based digital DNA–DNA hybridization (dDDH) values of 53.3 % and 15.3 %, compared with BCRC 81096 and . DSM 27345, respectively. ZX9611 had a genome size of 4.12 Mb and an average DNA G+C content of 64.8 %. ZX9611 had major fatty acids (>5 %) including summed feature 8 (C ω7 and/or C ω6), C 2-OH, C and summed feature 3 (C ω7 and/or C ω6), and the major polyamine was sym-homospermidine. The only respiratory quinone was ubiquinone-10. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and sphingoglycolipid. On the basis of phenotypic, chemotaxonomic and phylogenetic characteristics, strain ZX9611 represents a novel species of genus for which the name sp. nov. is proposed. The type strain is ZX9611 (=KCTC 72622=CCTCC AB 2019350).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): mountain soil , new taxa , polyphasic taxonomy and Sphingomonas montanisoli
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31670108)
    • Principle Award Recipient: Zixiao Xu
© 2020 Huazhong Agricultural University
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2020-05-14
2024-04-24
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Sphingomonas montanisoli sp. nov., isolated from mountain soil
Int J Syst Evol Microbiol 70, 3606 (2020); https://doi.org/10.1099/ijsem.0.004187
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