1887

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Volume 70, Issue 5

sp. nov., a novel endophytic actinobacterium isolated from roots of Free

Abstract

A novel endophytic actinobacterium, designated strain EGI 650086, was isolated from the roots of (C.A.Mey.) Schischk. collected in Xinjiang, north-west China. The taxonomic position of the strain was investigated using a polyphasic taxonomic approach. Growth occurred at 15–40 °C, pH 6.0–8.0 and in the presence of 0–6 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086 formed a monophyletic clade within the genus and shared the highest sequence similarities with JCM 14717 (97.1 %) and DSM 44468 (97.0 %). Sequence similarities with type strains of other species of the genus were less than 97.0 %. The average nucleotide identity and DNA–DNA hybridization values between strain EGI 650086 and the reference strains were 78.1–79.8 % and 22.1–23.0 %, respectively. The genome of strain EGI 650086 was 10.9 Mb, with a DNA G+C content of 70.1 mol%. The diagnostic diamino acid in the peptidoglycan was -diaminopimelic acid. The major whole-cell sugars contained arabinose, galactose, glucose and ribose. The predominant menaquinones were MK-9 (H) and MK-9 (H). Major fatty acids were -C and summed feature 4 (-C I and/or -C B). The polar lipid profile of strain EGI 650086 included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids, an unknown glycolipid and an unknown lipid. Polyphasic taxonomic characteristics indicated that strain EGI 650086 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EGI 650086 (=KCTC 49044=CGMCC 4.7188).

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  • Accepted:
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Keyword(s): Amycolatopsis anabasis sp. nov , Anabasis elatior and endophytic actinobacterium
Funding
This study was supported by the:
  • Department of Education of Liaoning Province (Award L201683673)
    • Principle Award Recipient: Hong-Fei Wang
  • Natural Science Foundation of Liaoning Province (Award 2019-ZD-0466)
    • Principle Award Recipient: Hong-Fei Wang
  • Key-Area Research and Development Program of Guangdong Province (Award 2018B020206001)
    • Principle Award Recipient: Wen-Jun Li
© 2020 Sun Yat-Sen University, P.R. China
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2020-05-04
2024-04-19
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Amycolatopsis anabasis sp. nov., a novel endophytic actinobacterium isolated from roots of Anabasis elatior
Int J Syst Evol Microbiol 70, 3391 (2020); https://doi.org/10.1099/ijsem.0.004184
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