1887

Abstract

Four strains, KC07070, KC07105, 11 025B-8C and 11 026B-8-C, were isolated from the oral cavity of cats in 2007 or 2011 in Japan. These strains were Gram-stain-negative rods, exhibited gliding motility, grew in air with 5 % CO and showed catalase and oxidase activity. The sequences of 16S rRNA genes of the four strains were 100 % identical. Additionally, the sequences of 16S rRNA genes of KC07070 had identity to those of the type strains of (97.7 %), (97.8 %) and (97.4 %) and 91.2–93.8% identity to those of other species of the genus . The major cellular fatty acids of KC07070 were iso-C (56.2 %) and summed feature 11 (14.9 %). The G+C content of the DNA from KC07070 was 35.6 mol%, and the genome size was 2.88 Mbp. KC07070 had digital DNA–DNA hybridization (dDDH) values of 26.2–27.6% and average nucleotide identity (ANI) values of 75.4–83.3 % to the type strains of the closest relatives, , and . These results of phylogenetic analysis of 16S rRNA gene sequence, cellular fatty acids compositions and dDDH and ANI values indicate that strain KC07070 represents a novel species, for which we propose the name sp. nov., with type strain KC07070 (=JCM 32681=DSM 107251).

Keyword(s): Capnocytophaga , feline and oral cavity
Funding
This study was supported by the:
  • Koichi Imaoka , Japan Agency for Medical Research and Development , (Award JP19fk0108069)
  • Koichi Imaoka , Japan Agency for Medical Research and Development , (Award JP17fk0108315)
  • Michio SUZUKI , Japan Society for the Promotion of Science , (Award JP15K08662)
  • Michio SUZUKI , Ministry of Health, Labour and Welfare , (Award H30-Shinkogyousei-Shitei-001)
  • Koichi Imaoka , Ministry of Health, Labour and Welfare , (Award H20-Shinko-Ippan-041)
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2020-04-28
2020-06-02
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