1887

Abstract

Two Gram-stain-negative, catalase- and oxidase-positive, rod-shaped, motile strains (FT29W and FT103W) were isolated from a subtropical stream in PR China. Comparisons based on 16S rRNA gene sequences showed that strains FT29W and FT103W showed 98.7 and 98.6 % 16S rRNA gene sequence similarities to CCM 3730 as their closest neighbour, respectively. The calculated pairwise OrthoANIu values between strain CCM 3730 and strains FT29W and FT103W were all 81.4 %. The respiratory quinone of strains FT29W and FT103W was determined to be Q-8. The major fatty acids were C ω7, C and C. The polar lipids of strain FT103W included phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid, but strain FT29W did not contain phosphatidylglycerol. The genome sizes of strains FT29W and FT103W were 7.23 and 6.84 Mbp with G+C contents of 63.8 and 63.9 %, respectively. Although the pairwise OrthoANIu value between strains FT29W and FT103W was 95.2 % which located in the transition region of species demarcation, the dissimilarities in the aspects of phenotypic, biochemical and genotypic characteristics supported these two strains should belong to the different species within genus , for which the names sp. nov. (type strain FT29W=GDMCC 1.1643=KACC 21316) and sp. nov. (type strain FT103W=GDMCC 1.1685=KACC 21477) are proposed.

Funding
This study was supported by the:
  • Meiying Xu , Guangdong MEPP Fund , (Award NO. GDOE(2019)A34)
  • Meiying Xu , GDAS’ Special Project of Science and Technology Development , (Award 2018GDASCX-0916)
  • Meiying Xu , National Natural Science Foundation of China , (Award 91851202, 51678163)
  • Meiying Xu , Guangdong Provincial Programs for Science and Technology Development , (Award 2019B110205004; 2018B020205003; 2018B030324002)
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2020-04-28
2020-06-04
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