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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 5

sp. nov., a novel actinobacterium isolated from rhizosphere soil of rice ( L.) Free

Abstract

A Gram-stain-positive, non-motile, creamy-white actinobacterium, which has an elementary branching rod-coccus life cycle was isolated from the rhizosphere soil of rice ( L.) collected from Northeast Agricultural University in Harbin, Heilongjiang province, north-east PR China, and its taxonomic status was examined by using a polyphasic approach. Results from the 16S rRNA gene sequence study showed that the isolate, designated strain NEAU-CX67, belonged to the genus and formed a cluster with DSM 44675, NEAU-ML12 and JCM 11308 (98.3, 98.1 and 97.7% gene sequence similarity, respectively). The major fatty acids were C, 10-methyl C, C ω9 and C ω7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major isoprenoid quinone was MK-8(H). Whole-cell hydrolysates contained -diaminopimelic acid. Arabinose, galactose and ribose were detected as diagnostic sugars from whole-cell hydrolysates. Mycolic acids were detected. The genomic DNA G+C content of strain NEAU-CX67 was 64.6 mol%. Strain NEAU-CX67 exhibited low average nucleotide identity and digital DNA–DNA hybridization values with DSM 44675 (92.1 and 45.4 %) and JCM 11308 (81.9 and 24.4 %). On the basis of results of phylogenetic, genotypic, physiological and chemotaxonomic analysis, strain NEAU-CX67 is considered to represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is NEAU-CX67 (=DSM 107701=CCTCC AB 2018233).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): 16S rRNA gene , polyphasic analysis , Rhodococcus oryzae sp. nov. and whole genome
Funding
This study was supported by the:
  • the National Natural Youth Science Foundation of China (Award 31701858)
    • Principle Award Recipient: Junwei Zhao
© 2020 The Authors
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2020-04-28
2024-04-24
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Rhodococcus oryzae sp. nov., a novel actinobacterium isolated from rhizosphere soil of rice (Oryza sativa L.)
Int J Syst Evol Microbiol 70, 3300 (2020); https://doi.org/10.1099/ijsem.0.004171
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