1887

Abstract

A chemolithoautotrophic sulfur-oxidizing bacterium, strain SGTM was isolated from snow collected in Japan. As electron donors for growth, SGTM oxidized thiosulfate, tetrathionate and elemental sulfur. Heterotrophic growth was not observed. Growth of the novel isolate was observed at a temperature range of 5–28 °C, with optimum growth at 18 °C. SGTM grew at a pH range of 4.3–7.4, with optimum growth at pH 6.1–7.1. Major components in the cellular fatty acid profile were summed feature 3 (Cω7 and/or Cω6) and C. The complete genome of SGTM consisted of a circular chromosome of approximately 3.4 Mbp and two plasmids. Phylogenetic analysis based on the 16S rRNA gene indicated that SGTM represented a member of the genus , and its closest relative is mst6 with a sequence identity of 98 %. A comparative genome analysis showed dissimilarity between the genomes of SGTM and mst6, as low values of average nucleotide identity (74.9 %) and digital DNA–DNA hybridization (20.4%). On the basis of its genomic and phenotypic properties, SGTM (=DSM 109609=BCRC 81185) is proposed as the type strain of a novel species, sp. nov. Some characteristics of another species in the same genus, , were also investigated to revise and supplement its description. The type strain of can grow on thiosulfate, tetrathionate and elemental sulfur. The strain showed optimum growth at pH 6.3–7.0 and shared major cellular fatty acids with the other species of the genus .

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2020-05-04
2024-03-28
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