1887

Abstract

A chemolithoautotrophic sulfur-oxidizing bacterium, strain SGTM was isolated from snow collected in Japan. As electron donors for growth, SGTM oxidized thiosulfate, tetrathionate and elemental sulfur. Heterotrophic growth was not observed. Growth of the novel isolate was observed at a temperature range of 5–28 °C, with optimum growth at 18 °C. SGTM grew at a pH range of 4.3–7.4, with optimum growth at pH 6.1–7.1. Major components in the cellular fatty acid profile were summed feature 3 (Cω7 and/or Cω6) and C. The complete genome of SGTM consisted of a circular chromosome of approximately 3.4 Mbp and two plasmids. Phylogenetic analysis based on the 16S rRNA gene indicated that SGTM represented a member of the genus , and its closest relative is mst6 with a sequence identity of 98 %. A comparative genome analysis showed dissimilarity between the genomes of SGTM and mst6, as low values of average nucleotide identity (74.9 %) and digital DNA–DNA hybridization (20.4%). On the basis of its genomic and phenotypic properties, SGTM (=DSM 109609=BCRC 81185) is proposed as the type strain of a novel species, sp. nov. Some characteristics of another species in the same genus, , were also investigated to revise and supplement its description. The type strain of can grow on thiosulfate, tetrathionate and elemental sulfur. The strain showed optimum growth at pH 6.3–7.0 and shared major cellular fatty acids with the other species of the genus .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004166
2020-05-04
2020-06-04
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/70/5/3273.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.004166&mimeType=html&fmt=ahah

References

  1. Watanabe T, Kojima H, Fukui M. Sulfuriferula multivorans gen. nov., sp. nov., isolated from a freshwater lake, reclassification of 'Thiobacillus plumbophilus' as Sulfuriferula plumbophilus sp. nov., and description of Sulfuricellaceae fam. nov. and Sulfuricellales ord. nov. Int J Syst Evol Microbiol 2015; 65:1504–1508 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  2. Drobner E, Huber H, Rachel R, Stetter KO. Thiobacillus plumbophilus spec. nov., a novel galena and hydrogen oxidizer. Arch Microbiol 1992; 157:213–217 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  3. Watanabe T, Kojima H, Fukui M. Sulfuriferula thiophila sp. nov., a chemolithoautotrophic sulfur-oxidizing bacterium, and correction of the name Sulfuriferula plumbophilus Watanabe, Kojima and Fukui 2015 to Sulfuriferula plumbiphila corrig. Int J Syst Evol Microbiol 2016; 66:2041–2045 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  4. Kojima H, Fukui M. Sulfuricella denitrificans gen. nov., sp. nov., a sulfur-oxidizing autotroph isolated from a freshwater lake. Int J Syst Evol Microbiol 2010; 60:2862–2866 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  5. Watanabe T, Kojima H, Shinohara A, Fukui M. Sulfurirhabdus autotrophica gen. nov., sp. nov., isolated from a freshwater lake. Int J Syst Evol Microbiol 2016; 66:113–117 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  6. Watanabe T, Kojima H, Umezawa K, Hori C, Takasuka TE et al. Genomes of neutrophilic sulfur-oxidizing chemolithoautotrophs representing 9 proteobacterial species from 8 genera. Front Microbiol 2019; 10:316 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  7. Widdel F, Bak F et al. Gram-negative mesophilic sulfate-reducing bacteria. In Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH et al. (editors) The Prokaryotes New York: Springer-Verlag; 1992 pp 3352–3378
    [Google Scholar]
  8. Fujii M, Kojima H, Iwata T, Urabe J, Fukui M. Dissolved organic carbon as major environmental factor affecting bacterioplankton communities in mountain lakes of eastern Japan. Microb Ecol 2012; 63:496–508 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  9. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35:1547–1549 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  10. Kojima H, Shinohara A, Fukui M. Sulfurifustis variabilis gen. nov., sp. nov., a sulfur oxidizer isolated from a lake, and proposal of Acidiferrobacteraceae fam. nov. and Acidiferrobacterales ord. nov. Int J Syst Evol Microbiol 2015; 65:3709–3713 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  11. Tanizawa Y, Fujisawa T, Nakamura Y. DFAST: a flexible prokaryotic genome annotation pipeline for faster genome publication. Bioinformatics 2017; 35:1037–1039
    [Google Scholar]
  12. Yoon S-H, Ha S-M, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281–1286 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  13. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60–14 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  14. Meyer B, Kuever J. Molecular analysis of the distribution and phylogeny of dissimilatory adenosine-5'-phosphosulfate reductase-encoding genes (aprBA) among sulfur-oxidizing prokaryotes. Microbiology 2007; 153:3478–3498 [CrossRef][PubMed][PubMed]
    [Google Scholar]
  15. Meier-Kolthoff JP, Klenk H-P, Göker M. Taxonomic use of DNA G+C content and DNA–DNA hybridization in the genomic age. Int J Syst Evol Microbiol 2014; 64:352–356 [CrossRef][PubMed][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004166
Loading
/content/journal/ijsem/10.1099/ijsem.0.004166
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error