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Abstract

A novel Gram-stain-positive, aerobic, non-motile actinobacterium, designated strain E2A, was isolated from a coral sample and examined using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E2A formed a distinct phyletic lineage in the genus and was closely related to CCTCC AA 2012022 (96.4 %) and CCTCC AA 2010012 (95.3 %). The isolate grew at 15–35 °C, pH 5–12 and in the presence of 1–16 % (w/v) NaCl. The cell-wall diamino acid was -DAP. Major fatty acids identified were -C, -C and C ω8c. The predominant menaquinone was MK-9(H). The polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmethylethanolamine, one unidentified glycolipid, one unidentified phospholipid and one unidentified aminolipid. The genomic DNA G+C content was 68.6 mol%. Based on the data from the polyphasic taxonomic study reported here, strain E2A represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is E2A=(JCM 31844=MCCC 1A17150).

Funding
This study was supported by the:
  • Gaiyun Zhang , the Scientific Research Foundation of Third Institute of Oceanography, MNR , (Award 2019011)
  • Gaiyun Zhang , China Ocean Mineral Resources R&D Association (COMRA) Program , (Award DY135-B2-01)
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2020-04-24
2020-06-02
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