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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 5

sp. nov., isolated from leaves of on the Qinghai–Tibet Plateau Free

Abstract

Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144 and 4053, were isolated from leaves of on the Qinghai–Tibet Plateau, PR China. Cells were aerobic, catalase-positive and oxidase-negative. Colonies on Reasoner’s 2A agar were light yellow, circular, shiny, smooth and convex after 2 days of incubation. The isolates grew optimally at 25 °C, pH 7.5 and with 0 % (w/v) NaCl. The results of polyphasic analyses indicated that strain S-1144 belonged to the genus and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were DSM 103718 (98.4 %), DSM 23986 (98.2%) and DSM 11054 (97.8 %). The genome of strain S-1144 showed less than 70 % digital DNA–DNA hybridization and < 95–96 % average nucleotide identity values to the above reference strains. The DNA G+C content of strain S-1144 was 73.5 mol%. MK-8(H) was the predominant respiratory quinone (96.0 %) and -2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The polar lipid profile of strain S-1144 consisted of diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, one unidentified glycolipid and one unidentified lipid. The major cellular fatty acids were iso-C, C 8, C and C 9. On the basis of obtained data, strain S-1144 represented a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S-1144 (=CGMCC 4.7568=JCM 33469).

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Keyword(s): leaves of Lamiophlomis rotata , Nocardioides dongxiaopingii sp. nov. and Qinghai–Tibet Plateau
Funding
This study was supported by the:
  • Research Units of Discovery of Unknown Bacteria and Function (Award 2018RU010)
    • Principle Award Recipient: Jianguo Xu
  • Sanming Project of Medicine in Shenzhen (Award SZSM201811071)
    • Principle Award Recipient: Jianguo Xu
  • National Key R&D Program of China (Award 2018YFC1200102)
    • Principle Award Recipient: Dong Jin
  • National Science and Technology Major Project of China (Award 2018ZX10712001-018)
    • Principle Award Recipient: Shan Lu
  • National Science and Technology Major Project of China (Award 2018ZX10712001-007)
    • Principle Award Recipient: Jing Yang
© 2020 The Authors
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2020-04-28
2024-04-25
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Nocardioides dongxiaopingii sp. nov., isolated from leaves of Lamiophlomis rotata on the Qinghai–Tibet Plateau
Int J Syst Evol Microbiol 70, 3234 (2020); https://doi.org/10.1099/ijsem.0.004161
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