@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004160, author = "Jiang, Hao and Han, Liyuan and Li, Jingjing and Yu, Mingying and Zhao, Junwei and Guo, Xiaowei and Xiang, Wensheng and Wang, Xiangjing", title = "Streptomyces montanus sp. nov., a novel actinomycete isolated from soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "5", pages = "3226-3233", doi = "https://doi.org/10.1099/ijsem.0.004160", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004160", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "16S rRNA gene", keywords = "polyphasic taxonomy", keywords = "Streptomyces montanus sp. nov.,", abstract = "A novel actinomycete, designated strain NEAU-C151T, was isolated from soil collected from Mount Song and characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence indicated that strain NEAU-C151T belongs to the genus Streptomyces and exhibited 97.5, 97.4 and 97.4 % similarities to Streptomyces lincolnensis NRRL 2936T, Streptomyces coacervatus AS-0823T, and Streptomyces longisporus ISP 5166T, respectively. The assignment of strain NEAU-C151T to the genus Streptomyces was confirmed by chemotaxonomic data: anteiso-C15 : 0, C16 : 0, iso-C16 : 0, C16 : 1 (ω7c) and anteiso-C17 : 0 as the major cellular fatty acids; whole-cell sugars contained ribose and glucose; phospholipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), unidentified phospholipid (PL), unidentified lipids (L) and phosphatidylinositol mannoside (PIM); the menaquinones were MK-9(H4), MK-9(H6), MK-10(H2) and MK-9(H8). However, multilocus sequence analysis based on five other house-keeping genes (atpD, gyrB, recA, rpoB, and trpB), DNA–DNA relatedness and phenotypic data showed that strain NEAU-C151T could be distinguished from its closest relatives. Consequently, strain NEAU-C151T represents a novel species of the genus Streptomyces , for which the name Streptomyces montanus sp. nov. is proposed. The type strain is NEAU-C151T (=CGMCC 4.7498T=DSM 107808T).", }