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Abstract

A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain, designated JLT9, was isolated from the shallow-sea hydrothermal system off Kueishantao Island, Taiwan, ROC. Strain JLT9 was aerobic, chemoheterotrophic and grew optimally at 35 °C, at pH 6.0 and in the presence of 2.5 % (w/v) NaCl. Strain JLT9 exhibited highest 16S rRNA gene sequence similarity to DSM 15273 (98.83 %). Phylogenetic trees based on 16S rRNA gene sequences revealed that strain JLT9 belonged to the genus , clustering with JC1078, MCCC 1A05965, GP-T3-3 and CAU9536. The digital DNA–DNA genome hybridization values between strain JLT9 and the closest related strain DSM 15273 was 34.30 %. The DNA G+C content was 72.43 mol%. The dominant fatty acids were identified as iso-C (41.4 %) and iso-C (24.7 %). The polar lipids of strain JLT9 comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, three unidentified glycolipid and an unidentified phospholipid. The predominant isoprenoid quinone was MK-8 (H). The cell wall contained ornithine and serine, and no diaminopimelic acid. On the basis of phylogenetic data and several distinct phenotypic characteristics, strain JLT9 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JLT9 (=CGMCC 1.15779=JCM 31502).

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2020-04-24
2020-06-02
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