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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 6

gen. nov., sp. nov., isolated from a marine solar saltern Free

Abstract

A Gram-stain-negative, non-motile, fine rod or short filament shaped, jacinth pigmented bacterium, designated strain WDS2C27, was isolated from a marine solar saltern in Wendeng, Weihai, PR China (37°31′5″ N, 122°1′47″ E). Growth of WDS2C27 occurred at 20–42 °C (optimum 37 °C) and pH 6.5–8.5 (optimal pH 7.0–8.0). Optimal growth occurred in modified marine broth containing 6 % (w/v) NaCl. The major polar lipids in WDS2C27 were phosphatidylethanolamine, two unidentified aminolipids and one unidentified lipid. The major respiratory quinone of WDS2C27 was MK-6. The dominant fatty acids were iso-C and anteisoC. The DNA G+C content was 35.0 mol%. The nucleotide sequence of the 16S rRNA gene indicated that the most closely related strain was X15M-8 (92.0 % over 1452 bp). WDS2C27 showed 60.7 % average amino acid identity, 55.6 % percentage of conserved proteins, 75.0 % average nucleotide identity and 13.1 % digital DNA–DNA hybridization identity with the type species of the genus , ATCC 700755. The phenotypic and genotypic properties and phylogenetic inference indicated that WDS2C27 could be assigned to a novel species within a novel genus, for which the name gen. nov., sp. nov. is proposed. Strain WDS2C27 (=MCCC 1H00133=KCTC 52044) is the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): draft genome sequencing , Mesohalobacter halotolerans and phylogenetic analysis
Funding
This study was supported by the:
  • the Science and Technology Basic Resources Investigation Program of China (Award 2017FY100300)
    • Principle Award Recipient: Zong-Jun Du
© 2020 The Authors
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2020-05-26
2023-11-30
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Mesohalobacter halotolerans gen. nov., sp. nov., isolated from a marine solar saltern
Int J Syst Evol Microbiol 70, 3588 (2020); https://doi.org/10.1099/ijsem.0.004143
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