1887

Abstract

is one of the genera that has high species diversity and , the type species of the genus, is mainly isolated from plant-associated environments. Among the plant-associated species, B.3 (=CECT 5266=DSM 13815=KACC 10925=KCTC 13919) was proposed to be a novel species according to 16S rRNA gene similarity and DNA–DNA relatedness with related species, including . Nevertheless, in the description of the used strain of was not the type strain of this species. In this work we found that the type strains of both species showed 16S rRNA gene similarity of 99.6 %. Therefore, in this study, we sequenced the genome of KACC 10925 and compared it with those of the type strain of ATCC 842 and other phylogenetically related species. Genome relatedness value calculated by DNA–DNA hybridization between type strains of and was 73.5 %, which is higher than the threshold value (70 %). For more objective and repeatable results of genome relatedness, we analysed an average nucleotide identity (ANI) between two strains. Our results showed that ANI value between the type strains of and is 98.5 %, a phylogenetic distance also higher than the threshold values (95~96 %). These values were proposed by Yoon . (2017). In addition, their phylogenetic distance based on 92 bacterial core genes is highly close compared to other species. These mean that and should be reclassified as a single species. Based on the results from genomic level comparison as well as reexamination results of physiological and chemotaxonomic features, we propose reclassification of as a later heterotypic synonym of .

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2020-04-24
2020-06-04
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