1887

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Volume 70, Issue 5

sp. nov. (formerly ‘’), an actinomycete isolated from soil which produces the polyether antibiotic lasalocid Free

Abstract

Strain ATCC 31180 was isolated from soil collected in Hyde Park, Massachusetts (USA), and found to produce the polyether antibiotic lasalocid. The name ‘’ has been in common use since 1974, without a recognized taxonomic description. The most closely related type cultures determined by rRNA gene sequence similarity were DSM 41677 (100 %) and DSM 40089 (100 %). OrthoANI values with and were 95.50 and 94.41 %, respectively. Chemotaxonomic characteristics supported inclusion within the genus . The cell wall peptidoglycan contained -diaminopimelic acid, and the major whole-cell sugars were glucose and ribose. Polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol, one unidentified lipid and one unidentified glycolipid. The major menaquinones detected were MK9(H), MK9(H) and MK9(H). The major cellular fatty acids were anteisoC, anteisoC, isoC, isoC and anteisoC. Its DNA had a G+C content of 72.6 %. Differentiation of ATCC 31180 from the closely related species was evident from digital DNA–DNA hybridization values of 61.80 and 56.90 % for and respectively. Significant differences were seen in the polyphasic phenotypic analyses. ATCC 31180 produced lasalocid, grew from 10 to 45 °C, pH4-8 and in the presence of 0–10 % NaCl, 0.01 % NaN and 1 % phenol. Melanin was produced; HS and indole were not. Nitrate was not reduced. Spore chains were retinaculum-apertum and spore surfaces were smooth. Spore colour, mycelia colour and soluble pigment production were medium-dependent. The proposed name is sp. nov.; the type strain being ATCC 31180 (=NRRL 3382=DSM 46487).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): lasaliensis , lasalocid , lasalocidi , polyether , Streptomyces and taxonomy
Funding
This study was supported by the:
  • Zoetis (US)
    • Principle Award Recipient: Not Applicable
© 2020 Zoetis Services LLC
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2020-03-31
2024-04-19
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Streptomyces lasalocidi sp. nov. (formerly ‘Streptomyces lasaliensis’), an actinomycete isolated from soil which produces the polyether antibiotic lasalocid
Int J Syst Evol Microbiol 70, 3076 (2020); https://doi.org/10.1099/ijsem.0.004135
/content/journal/ijsem/10.1099/ijsem.0.004135
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