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Volume 70, Issue 5

sp. nov., isolated from a Yellow River sample Free

Abstract

A novel actinobacterium, designated strain CFH S0261, was isolated from a sediment sample of the Yellow River. The taxonomic position of the strain was investigated by using a polyphasic approach. Cells of strain CFH S0261 were Gram-reaction-positive, aerobic, non-motile. Growth occurs at 15–37 °C, pH 6.0–8.0 and with 0–9.0 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CFH S0261 was a member of the genus . The 16S rRNA gene sequence similarity indicated that strain CFH S0261 is most closely related to the type strains of LC11 (98.95 %), LC2 (98.81 %) and IMSNU 22139 (98.73 %). The whole-genome of CFH S0261 showed a G+C content of 69.5 mol%. The ANI values and DDH values between CFH S0261 and the other species of the genus were found to be low (ANIb <90.61 % and DDH <53.40 %). The cell wall diamino acid in the peptidoglycan of strain CFH S0261 was -diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose. The predominant menaquinone was MK-9(H). The major cellular fatty acids were C, iso-C and iso-C. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and four unidentified glycolipids. On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CFH S0261 (=KCTC 39515 =CCTCC AA 2014028).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Amycolatopsis niveasp. nov , polyphasic taxonomy and Yellow River
Funding
This study was supported by the:
  • Doctor Scientific Research Fund of Xinxiang Medical University (Award XYBSKYZZ201625)
    • Principle Award Recipient: Hong Ming
  • Henan Province University youth researcher support project (Award 2017GGJS106)
    • Principle Award Recipient: Hong Ming
  • Innovation Scientists and Technicians Troop Construction Projects of Henan Province (Award CXTD2016043)
    • Principle Award Recipient: Guo-Xing Nie
  • Natural Science Foundation of China (Award 3150004)
    • Principle Award Recipient: Hong Ming
© 2020 The Authors
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2020-04-06
2024-04-25
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Amycolatopsis nivea sp. nov., isolated from a Yellow River sample
Int J Syst Evol Microbiol 70, 3084 (2020); https://doi.org/10.1099/ijsem.0.004134
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