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Volume 70, Issue 5

sp. nov., a novel actinobacterium isolated from rhizosphere soil of wheat ( L.) Free

Abstract

A novel actinobacterium, designated strain NEAU-7082, was isolated from rhizosphere soil of wheat ( L.) and characterized by using a polyphasic approach. Morphological and chemotaxonomic characteristics confirmed the affiliation of strain NEAU-7082 to the genus . 16S rRNA gene sequence analysis indicated that strain NEAU-7082 belonged to the genus and was closely related to JCM 16217 (99.0 % 16S rRNA gene sequence similarity), DSM 45047 (98.4 %), DSM 44968 (98.3 %), DSM 102295 (98.1 %), NBRC 109773 (98.0 %) and DSM 105121T (97.9 %). Phylogenetic analysis using the 16S rRNA gene sequence showed that the strain formed a stable clade with JCM 16217 and clustered with DSM 45047, DSM 44968, NBRC 109773 and DSM 105121T in the genus . The cell wall contained -diaminopimelic acid and the whole-cell hydrolysates were glucose and xylose. The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), glycolipid (GL), phosphatidylinositol mannoside (PIM) and an unidentified lipid (UL). The menaquinones were MK-11(H), MK-11 and MK-10. Major fatty acids were anteiso-C, iso-C and anteiso-C. These chemotaxonomic data substantiated the affiliation of strain NEAU-7082 to the genus . The DNA G+C content was 71.3 mol%. A combination of DNA–DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-7082 could be distinguished from its closest relatives. Therefore, strain NEAU-7082 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-7082 (=CCTCC AA 2019045=JCM 33458).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): 16S rRNA gene , Glycomyces albidus sp. nov and polyphasic taxonomy
Funding
This study was supported by the:
  • the National Key Research and Development Program of China (Award 2017YFD0201606)
    • Principle Award Recipient: Xiangjing Wang
© 2020 The Authors
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2020-04-03
2024-04-19
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Glycomyces albidus sp. nov., a novel actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.)
Int J Syst Evol Microbiol 70, 3096 (2020); https://doi.org/10.1099/ijsem.0.004131
/content/journal/ijsem/10.1099/ijsem.0.004131
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