1887

Abstract

A Gram-stain-negative, aerobic, rod-shaped and motile bacterial strain, designated L53-10-65, was isolated from deep seawater of the South China Sea. Strain L53-10-65 was found to grow at 4–41 °C (optimum, 28 °C), at pH 5.0–9.0 (pH 7.0–8.0) and in 0–7 % (w/v) NaCl (2 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain L53-10-65 fell within the genus , with the highest similarity to IO390501 (98.4 %), followed by ‘’ L15 (97.7 %) and IFO 13584 (96.8 %). The digital DNA–DNA hybridization (dDDH) values between strain L53-10-65 and the three relatives above were 43.7, 21.5 and 20.9 %, respectively; the average nucleotide identity (ANI) values were 91.3, 78.4 and 76.8 %, respectively. These values were below the 70 % dDDH and 95–96 % ANI thresholds for bacterial species delineation. The major cellular fatty acids of strain L53-10-65 were summed feature 8 (C 7 and/or C 6), C 7 11-methyl and C. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and glycolipids. The respiratory quinone was identified as Q-10. The DNA G+C content of strain L53-10-65 was 61.3 mol%. Based on the results of phylogenetic analysis and distinctive phenotypic characteristics, strain L53-10-65 is concluded to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of the species is L53-10-65 (=MCCC 1A05139=KCTC 72888). Moreover, we propose that is a later heterotypic synonym of based on the present results.

Funding
This study was supported by the:
  • the Science and Technology Plan Project of Guangdong Province (Award 2019B030316017)
    • Principle Award Recipient: Yang Liu
  • the GDAS' Special Project of Science and Technology Development (Award 2020GDASYL-20200302002)
    • Principle Award Recipient: Honghui Zhu
  • the Key-Area Research and Development Program of Guangdong Province (Award 2018B020205003)
    • Principle Award Recipient: Honghui Zhu
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2020-04-28
2024-03-28
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