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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 7

sp. nov., isolated from a cave, and is a later heterosynonym of Free

Abstract

A Gram-reaction-positive, strictly aerobic, catalase-positive, oxidase-negative, non-motile actinobacterium, designated C1-24, was isolated from a soil sample collected inside a natural cave. The organism exhibited a rod–coccus developmental cycle during its growth phase. Results of 16S rRNA gene-based phylogenetic analysis showed that the novel strain belonged to the genus and formed a distinct sublineage at the base of the radiation including a cluster. In the results of phylogenomic analysis, the novel strain was loosely associated to . The closest relatives were (98.01 % 16S rRNA gene sequence similarity) and (98.01 %). The genome size was 5.66 Mbp and the DNA G+C content was 64.30 mol%. Whole-cell hydrolysates contained -diaminopimelic acid, arabinose and galactose as the diagnostic diamino acid and sugars. MK-8(H) was the predominant menaquinone. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, an unidentified glycolipid and three unidentified phospholipids. Mycolic acids were present. The major fatty acids were C, C ω9, C ω7 and/or C ω6 and 10-methyl C. Digital DNA–DNA hybridization and average nucleotide identity values revealed that the novel strain should be assigned to a different species. Based on the combined data obtained here, strain C1-24 (=KACC 19964=DSM 109484) represents a new species of the genus , for which sp. nov. is proposed. Also, it is proposed that is a later heterosynonym of based on analyses of 16S rRNA gene and whole-genome sequences.

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  • Accepted:
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Keyword(s): benzoyl CoA catabolism , biosynthesis of secondary metabolite , cave , mevalonate pathway and Rhodococcus cavernicola
© 2020 The Authors
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2020-06-15
2025-01-26
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Rhodococcus cavernicola sp. nov., isolated from a cave, and Rhodococcus degradans is a later heterosynonym of Rhodococcus qingshengii
Int J Syst Evol Microbiol 70, 4409 (2020); https://doi.org/10.1099/ijsem.0.004126
/content/journal/ijsem/10.1099/ijsem.0.004126
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