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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 5

sp. nov., a pathogen causing bacterial rot of Welsh onion in Japan Free

Abstract

Five Gram-reaction-negative, aerobic, motile with one to three polar flagella, rod-shaped bacterial strains, MAFF 212408, MAFF 212409, MAFF 212410, MAFF 301498 and MAFF 730085, were isolated from diseased Welsh onion ( L.) in Japan. Analysis of their 16S rRNA gene sequences showed that they belong to the genus with the highest similarity to 14-3 (99.86 %), CMS 35 (99.79 %) and DSM 14936 (99.72%). The genomic DNA G+C content was 59.5 mol% and the major fatty acids (>5 %) were summed feature 3, C, summed feature 8 and C 2-OH. Multilocus sequence analysis using the , and gene sequences and phylogenomic analysis based on the 90 core genes demonstrated that the strains are members of the subgroup, but are distant from all closely related species. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) analysis confirmed low genomic relatedness to their closest relatives [below the recommended thresholds of 95 % (ANI) and 70 % (dDDH) for prokaryotic species delineation]. The strains were characterized by using API 20NE and Biolog GEN III tests, and inoculation tests in Welsh onion, showing that they are phenotypically differentiated from their closest relatives. Based on the genetic and phenotypic evidence, the strains should be classified as representing a novel species, for which the name sp. nov. is proposed. The type strain is MAFF 212408 (=ICMP 23530).

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Keyword(s): Allium fistulosum , bacterial rot disease , phylogenomic analysis , polyphasic taxonomy , Pseudomonas kitaguniensis and Welsh onion
© 2020 The Authors
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2020-03-31
2024-04-25
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Pseudomonas kitaguniensis sp. nov., a pathogen causing bacterial rot of Welsh onion in Japan
Int J Syst Evol Microbiol 70, 3018 (2020); https://doi.org/10.1099/ijsem.0.004123
/content/journal/ijsem/10.1099/ijsem.0.004123
/content/journal/ijsem/10.1099/ijsem.0.004123
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