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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 5

, sp. nov., isolated from offshore sediment Free

Abstract

An aerobic, Gram-stain-negative bacterium, designated CLL7-20, was isolated from a marine sediment sample from offshore of Changyi, Shandong Province, China. Cells of strain CLL7-20 were rod-shaped, motile with one or more polar flagella, and grew optimally at pH 7.0, at 28 °C and with 3 % (w/v) NaCl. The principal fatty acids of strain CLL7-20 were C and summed feature 3 (C 7/C 6). The main polar lipids of strain CLL7-20 were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and an unidentified aminolipid (AL). Strain CLL7-20 contained Q-9 as the major respiratory quinone. The G+C content of its genomic DNA was 56.2 mol%. Phylogenetically, strain CLL7-20 branched within the genus , with YCSA40 being its closest phylogenetic relative (96.7 % 16S rRNA gene sequence similarity), followed by R65 (96.6 %). Average nucleotide identity and DNA–DNA hybridization values between strain CLL7-20 and the closest related reference strains were 73.2% and 19.8 %, respectively. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, we suggest that strain CLL7-20 (=MCCC 1A14855=KCTC 72664) is the type strain of a novel species in the genus , for which the name sp. nov. is proposed. Based on the genomic analysis, siderophore genes were found from strain CLL7-20, which indicate its potential as a promising alternative to chemical fertilizers in iron-limitated environments such as saline soils.

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Keyword(s): Marinobacter changyiensis sp. nov. and offshore sediment
Funding
This study was supported by the:
  • National Infrastructure for Microbiological Resources (Award NIMR-2019-9)
    • Principle Award Recipient: Zongze Shao
  • China Ocean Mineral Resources Research and Development Association (CN) (Award DY135-B2-01)
    • Principle Award Recipient: Zongze Shao
© 2020 The Authors
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2020-04-22
2024-04-25
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Marinobacter changyiensis, sp. nov., isolated from offshore sediment
Int J Syst Evol Microbiol 70, 3004 (2020); https://doi.org/10.1099/ijsem.0.004118
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