RT Journal Article SR Electronic(1) A1 Feng, Guang-Da A1 Chen, Wendi A1 Zhang, Xian-Jiao A1 Zhang, Jun A1 Wang, Sheng-Nan A1 Zhu, HonghuiYR 2020 T1 Methylobacterium nonmethylotrophicum sp. nov., isolated from tungsten mine tailing JF International Journal of Systematic and Evolutionary Microbiology, VO 70 IS 4 SP 2867 OP 2872 DO https://doi.org/10.1099/ijsem.0.004112 PB Microbiology Society, SN 1466-5034, AB A novel pink-pigmented strain, designated 6HR-1T, was isolated from tungsten mine tailings in Jiangxi Province, PR China. Cells were Gram-stain-negative, aerobic, non-spore-forming, rod-shaped and motile with a polar flagellum (monotrichous). It could not utilize methanol, methylamine, formaldehyde or formate as a sole carbon source. The methanol dehydrogenase mxaF gene was absent but the xoxF gene was present. Phylogenomic and 16S rRNA gene phylogenetic analyses clearly showed that strain 6HR-1T was affiliated to the genus Methylobacterium and closely related to ‘Methylobacterium terrae’ 17Sr1-28T (98.6 %), Methylobacterium platani JCM 14648T (97.7 %), Methylobacterium variabile DSM 16961T (97.7 %) and Methylobacterium currus KACC 19662T (97.4 %). The average nucleotide identity and digital DNA–DNA hybridization values between strain 6HR-1T and its closely related type species were 87.4–88.7 and 33.2–36.3 %, respectively. It had summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) as the major fatty acid and ubiquinone 10 as the predominant respiratory quinone. Polyphasic characterization supported that strain 6HR-1T represents a novel species of the genus Methylobacterium , for which the name Methylobacterium nonmethylotrophicum sp. nov. is proposed with the type strain 6HR-1T (=GDMCC 1.662T=KCTC 42760T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004112