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Volume 70, Issue 4

sp. nov., isolated from tungsten mine tailing Free

Abstract

A novel pink-pigmented strain, designated 6HR-1, was isolated from tungsten mine tailings in Jiangxi Province, PR China. Cells were Gram-stain-negative, aerobic, non-spore-forming, rod-shaped and motile with a polar flagellum (monotrichous). It could not utilize methanol, methylamine, formaldehyde or formate as a sole carbon source. The methanol dehydrogenase gene was absent but the gene was present. Phylogenomic and 16S rRNA gene phylogenetic analyses clearly showed that strain 6HR-1 was affiliated to the genus and closely related to ‘’ 17Sr1-28 (98.6 %), JCM 14648 (97.7 %), DSM 16961 (97.7 %) and KACC 19662 (97.4 %). The average nucleotide identity and digital DNA–DNA hybridization values between strain 6HR-1 and its closely related type species were 87.4–88.7 and 33.2–36.3 %, respectively. It had summed feature 8 (C ω7 and/or C ω6) as the major fatty acid and ubiquinone 10 as the predominant respiratory quinone. Polyphasic characterization supported that strain 6HR-1 represents a novel species of the genus , for which the name sp. nov. is proposed with the type strain 6HR-1 (=GDMCC 1.662=KCTC 42760).

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  • Accepted:
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  • Published Online:
Keyword(s): Methylobacterium nonmethylotrophicum , tungsten mine tailing and whole genome sequence
Funding
This study was supported by the:
  • the GDAS' Special Project of Science and Technology Development (Award 2020GDASYL-20200302002)
    • Principle Award Recipient: Honghui Zhu
  • the Science and Technology Programs of Guangdong Province (Award 2018B030324001)
    • Principle Award Recipient: Guang-Da Feng
  • the Key Realm R&D Program of Guangdong Province (Award 2018B020205001)
    • Principle Award Recipient: Guang-Da Feng
© 2020 The Authors
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2024-04-19
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Methylobacterium nonmethylotrophicum sp. nov., isolated from tungsten mine tailing
Int J Syst Evol Microbiol 70, 2867 (2020); https://doi.org/10.1099/ijsem.0.004112
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